Acquisition of a CombiFlash EZ Prep Chromatography System with Integrated ELSD for Chemical Synthesis

采购具有集成 ELSD 的 CombiFlash EZ Prep 色谱系统，用于化学合成

• 批准号: 10792186
• 负责人: JOHN A PORCO
• 金额: $ 6.89万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10792186
• 关键词: Boston; Chromatography; Collection; Complex; Contract Services; Goals; High Pressure Liquid Chromatography; Human; Institution; Laboratories; Malignant Neoplasms; Natural Products; Phase; Research; Solvents; Synthesis Chemistry; System; Translational Research; Universities; Viral; analog; chemical synthesis; cost; detector; flexibility; instrument; light scattering; multitask; pharmacologic; programs; translational study

PROJECT SUMMARY Acquisition of a CombiFlash EZ Prep Chromatography System with Integrated ELSD for Chemical Synthesis The goals of the continuing R35GM118173 research program “Chemical Synthesis of Complex Natural Products for Translational Science” involve chemical syntheses of bioactive molecules for translational studies. An instrument supplement is requested to purchase a Teledyne ISCO Combiflash EZ Prep Chromatography System with Integrated Evaporative Light Scattering Detector (ELSD) to establish a highly flexible and complete solution for both normal and reverse-phase purifications of biologically active natural products and analogues as well as synthetic intermediates in the Porco laboratory at Boston University. The instrument would replace a dysfunctional prep-HPLC system to enable and expedite research in many ways, as compound purification is a substantial aspect of the research workflow. Automated solvent fraction collection would also provide a significant advantage by expediting purifications and enabling increased multitasking and greater focus on synthetic chemistry. As part of Boston University’s Institutional Support, the Provost Office has committed to the cost of a service contract from Teledyne Isco to support the chromatography system.

项目摘要 用于化学分析的集成ELSD的CombiFlash EZ制备色谱系统的购置 合成 R35GM118173研究项目“复杂天然产物的化学合成”的目标是： 转化科学产品”涉及转化研究用生物活性分子的化学合成。 购买Teledyne ISCO Combiflash EZ Prep Chromatography需要补充仪器 集成蒸发光散射检测器（ELSD）的系统，以建立高度灵活且 生物活性天然产物的正相和反相纯化的完整解决方案， 类似物以及合成中间体在波斯顿大学的Porco实验室。仪器 将取代功能失调的制备型HPLC系统，以在许多方面实现和加快研究， 化合物纯化是研究工作流程的重要方面。自动溶剂级分收集 还将通过加速净化和实现增加的多任务处理提供显著的优势， 更加注重合成化学。作为波士顿大学机构支持的一部分，教务长办公室 已承诺支付Teledyne Isco的服务合同费用，以支持色谱系统。

项目成果

Biomimetic Total Synthesis of (±)-Griffipavixanthone via a Cationic Cycloaddition-Cyclization Cascade.

• DOI: 10.1021/jacs.7b09265
• 发表时间: 2017-10-11
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Reichl KD;Smith MJ;Song MK;Johnson RP;Porco JA Jr
• 通讯作者: Porco JA Jr

Total Syntheses of the Isomeric Aglain Natural Products Foveoglin A and Perviridisin B: Selective Excited-State Intramolecular Proton-Transfer Photocycloaddition.

• DOI: 10.1002/anie.201707539
• 发表时间: 2017-11-13
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: Wang W;Clay A;Krishnan R;Lajkiewicz NJ;Brown LE;Sivaguru J;Porco JA Jr
• 通讯作者: Porco JA Jr

Eucalyptusdimers A-C, Dimeric Phloroglucinol-Phellandrene Meroterpenoids from Eucalyptus robusta.

• DOI: 10.1021/acs.orglett.8b02259
• 发表时间: 2018-08-17
• 期刊: Organic letters
• 影响因子: 5.2
• 作者: Qin XJ;Feng MY;Liu H;Ni W;Rauwolf T;Porco JA Jr;Yan H;He L;Liu HY
• 通讯作者: Liu HY

Syntheses of (+)-30-epi-, (-)-6-epi-, (±)-6,30-epi-13,14-Didehydroxyisogarcinol and (±)-6,30-epi-Garcimultiflorone A Utilizing Highly Diastereoselective, Lewis Acid-Controlled Cyclizations.

• DOI: 10.1021/jacs.6b09727
• 发表时间: 2016-11-09
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Boyce JH;Eschenbrenner-Lux V;Porco JA Jr
• 通讯作者: Porco JA Jr

Inhibition of the Translation Initiation Factor eIF4A Enhances Tumor Cell Radiosensitivity.

• DOI: 10.1158/1535-7163.mct-22-0037
• 发表时间: 2022-09-06
• 期刊: Molecular cancer therapeutics
• 影响因子: 5.7