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National Biomedical NMR Resource at Madison

麦迪逊国家生物医学 NMR 资源

基本信息

批准号：
8316486
负责人：
JOHN LUTE MARKLEY
金额：
$ 19.6万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-03-01 至 2015-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8316486
关键词：
Area Automation Automobile Driving Binding Biological Biological Factors Biomolecular Nuclear Magnetic Resonance Cell Extracts Chemicals Communities Complex Computer software Consult Data Data Analyses Data Collection Databases Deposition Documentation Educational workshop Environmental Risk Factor Evaluation Feasibility Studies Genetic Goals Health Human Human Resources Instruction Investigation Knowledge Label Liquid Chromatography Liquid substance Magnetic Resonance Manuscripts Membrane Proteins Methodology Methods Modeling NMR Spectroscopy Newsletter Nuclear Magnetic Resonance Nucleic Acids Pharmaceutical Preparations Physiologic pulse Preparation Process Production Proteins Protocols documentation Publications Qualifying RNA Recovery Research Research Activity Research Personnel Resources Sampling Scientist Screening procedure Services Site Software Tools Solutions Structure System Technology Tissues Training Validation Visit Work base biological systems cost data structure design experience frontier high throughput analysis instrumentation lectures meetings metabolomics molecular dynamics new technology novel posters programs protein complex protein structure research and development research study small molecule software development technology development three dimensional structure tool web site

项目摘要

The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource for biomolecular nuclear magnetic resonance (NMR) spectroscopy. NMRFAM aims to expand the frontiers of biomolecular NMR spectroscopy through resource technology and development programs in the important areas of (1) highthroughput determination of structures and functions of smaller proteins and RNA molecules, (2) technology for investigating the structure and dynamics of challenging systems, including complexes, membrane proteins, paramagnetic proteins, and larger RNA molecules, and (3) efficient approaches to metabolomics and natural product analysis. NMRFAM strives to be a model to the larger biological community for cutting-edge capabilities of NMR spectroscopy. With the goal of broadening the scope of its scientific activities, NMRFAM hosts distinguished visiting scientists working in areas related to its research technology and development projects. NMRFAM develops and disseminates advanced approaches that cover all steps in biomolecular NMR investigations and offers start-to-finish support for biomedical NMR investigations with assistance in one or more of the following steps: (1) strategy evaluation and experiment design, (2) sample preparaton, (3) feasibility studies, (4) data collection, (5) data analysis and structure determination, (6) data validation and deposition, and (7) manuscript preparation. NMRFAM provides researchers with resources matched to their particular needs in their search for new knowledge. A major goal is to develop methods for making these investigations faster and less costly as well as applicable to larger classes of proteins and nucleic acids of importance in human health. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation with support for multiple modes of data collection either as service or collaborative projects. Protocols, pulse sequences, software tools, and databases developed through NMRFAM's research activities are made available to the general scientific community. Users receive hands-on training at the center. As another means for training its user base and for disseminating its novel technology, NMRFAM conducts annual workshops and group training sessions. Additional mechanisms used to disseminate NMRFAM technology include quarterly newsletters, the NMRFAM website, software servers, a metabolomics database, presentations at meetings, and the publication of articles and reviews.

麦迪逊国家磁共振设施（NMRFAM）是生物分子核的一个资源

项目成果

期刊论文数量（431）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Multinuclear magnetic resonance studies of the 2Fe.2S* ferredoxin from Anabaena species strain PCC 7120. 2. Sequence-specific carbon-13 and nitrogen-15 resonance assignments of the oxidized form.

来自鱼腥藻菌株 PCC 7120 的 2Fe.2S* 铁氧还蛋白的多核磁共振研究。2.氧化形式的序列特异性碳 13 和氮 15 共振归属。

DOI：
10.1021/bi00468a030
发表时间：
1990
期刊：
Biochemistry
影响因子：
2.9
作者：
Oh,BH;Mooberry,ES;Markley,JL
通讯作者：
Markley,JL

Recombinant Expression, Isotope Labeling and Purification of the Vitamin D Receptor Binding Peptide.

维生素 D 受体结合肽的重组表达、同位素标记和纯化。

DOI：
10.5012/bkcs.2011.32.12.4337
发表时间：
2011
期刊：
Bulletin of the Korean Chemical Society
影响因子：
1.7
作者：
Chae,YoungKee;Singarapu,Kiran;Westler,WMilo;Markley,JohnL
通讯作者：
Markley,JohnL

Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies.

有效的稳定同位素标记和从包涵体中纯化维生素 D 受体。

DOI：
10.1016/j.pep.2012.06.012
发表时间：
2012
期刊：
Protein expression and purification
影响因子：
1.6
作者：
Zhu,Jinge;Rao,Hongyu;Tonelli,Marco;Westler,WilliamM;Singarapu,KiranK;Markley,JohnL;DeLuca,HectorF;Assadi-Porter,FaribaM
通讯作者：
Assadi-Porter,FaribaM

13C NMR analysis of electrostatic interactions between NAD+ and active site residues of UDP-galactose 4-epimerase: implications for the activation induced by uridine nucleotides.

NAD 和 UDP-半乳糖 4-差向异构酶活性位点残基之间的静电相互作用的 13C NMR 分析：对尿苷核苷酸诱导的激活的影响。