Viper Resource Grant at Texas A&M University-Kingsville

NNTRC的应用研究计划将提供资源，以支持改善 NNTRC向其用户社区提供的产品和服务。这些努力将侧重于以下领域： 产品和服务的改进可以对毒液产生最重大的影响- 相关研究。在下一个资助期内，应用研究计划将集中在两个非常具体的领域： 1)应用新方法和途径生产具有医学意义的重组 蛇毒毒素和2）新的细胞为基础的测定，可用于测试抗- 毒液和毒素抑制分子。 在下一个资助期内，其中一个重点是改善制作 重组蛇毒毒素，以满足对重组毒素日益增长的需求， 制药和抗毒血清研究NNTRC将开展一项应用研究计划， 目标是扩大和提高NNTRC生产医学上重要的重组毒液的能力 proteins.虽然一些毒液毒素相对容易使用传统的分子技术生产， 许多其它的化合物由于不溶性、广泛的分子内交联 和翻译后修饰。重组蛋白生产领域的最新进展 打开了应用新方法来提高“柠檬酸盐”重组体生产的大门， proteins.在下一个赠款期内，NNTRC将探索使用这些新方法来改善 该中心生产广谱重组蛇毒毒素的能力。 此外，在下一个资助期内，NNTRC将对 开发和验证新的体外筛选测定法，其可用于分析新化合物的活性， 蛇毒解毒剂。这些基于细胞的测定不仅会减少动物毒性的使用 测试，但也将提供专门的研究服务，以支持发现和优化下一个 一代蛇毒解毒剂！

The NNTRC's Applied Research Program will provide resources to support the improvement of the products and services that the NNTRC provides to its user community. These efforts will be focused on areas of research where the improvement in products and services can have the most significant impact on venom- related research. In the next grant period, the Applied Research Program will focus on two very specific areas: 1) the application of new methods and approaches to the production of medically significant recombinant snake venom toxins and 2) the development of novel cell-based assays that can be used for the testing of anti- venoms and toxin inhibitory molecules. One of the major areas of emphasis in the coming grant period will be improvements in the production of recombinant snake venom toxins to address the growing demand for recombinant toxins to support both pharmaceutical and anti-venom research. The NNTRC will conduct a program of applied research with the goal of expanding and improving the NNTRC's capacity to produce medically important recombinant venom proteins. While some venom toxins are relatively easy to produce using conventional molecular technologies, many others are difficult to produce in an active form due to insolubility, extensive intramolecular cross-linking and post-translational modifications. Recent advances in the field of recombinant protein production has opened the door to the application of new approaches to improving the production of “recalcitrant” recombinant proteins. In the next grant period, the NNTRC will explore the use of these new methods to improve the Center's ability to produce a broad spectrum of recombinant snake venom toxins. In addition, during the next funding period, the NNTRC will conduct applied research on the development and validation of new in vitro screening assays that can be used to profile the activity of novel snake venom toxin antidotes. These cell-based assays will not only reduce the use of animals for toxicity testing but will also provide specialized research services to support the discovery and optimization of the next generation of snake venom antidotes.!