Viper Resource Center (VRC) at Texas A&M University-Kingsville

位于德克萨斯州 A 的 Viper 资源中心 (VRC)

• 批准号: 8241954
• 负责人: Elda E. Sanchez
• 金额: $ 48.96万
• 依托单位: TEXAS A&M UNIVERSITY-KINGSVILLE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8241954
• 关键词: Adhesions; Affect; Alleles; Alternative Splicing; Amino Acid Sequence; Animals; Applications Grants; Area; Biological Assay; Biomedical Research; Blood Cells; Blood Platelets; Breeding; Cause of Death; Cell physiology; Cells; Cessation of life; Coagulation Process; Communities; Complex Mixtures; Databases; Development; Disease; Disintegrins; Dose; Evaluation; Fibrinogen; Funding; Future; Genetic Variation; Geographic Locations; Goals; Hemorrhage; High Pressure Liquid Chromatography; Information Dissemination; Inhibitory Concentration 50; Internet; Lead; Lethal Dose 50; Malignant Neoplasms; Medical; Medicine; Mission; Molecular Weight; Myocardial Infarction; Pharmaceutical Preparations; Platelet Aggregation Inhibition; Population; Procedures; Process; Property; Proteins; Proteomics; Protocols documentation; Publications; Research; Research Personnel; Research Training; Resources; Sampling; Signal Transduction; Snake Venoms; Snakes; Source; Standardization; Stroke; Techniques; Texas; Therapeutic; Time; Titrations; Toxin; United States; United States National Institutes of Health; Universities; Variant; Venoms; base; biomedical resource; data sharing; database design; enzyme activity; improved; inhibitor/antagonist; instrumentation; prevent; programs; success; web site

DESCRIPTION (provided by applicant): The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application. The resources will be expanded and snake venom, venom fractions, blood and cells will be made available to the biomedical research community. The research segment of the proposal will focus on improving the products by characterizing important molecules found in venom for biomedical research. Because of university support and NIH funding, the VRC within the Natural Toxins Research Center (NTRC) has developed a unique facility with the state-of-the-art instrumentation. There are 300 different species of venomous snakes in the world and venoms are complex mixtures of thousands of molecules. Many venomous snakes use their venoms for capturing prey by interfering with the clotting cascade, which causes the animal to bleed to death. These same molecules could have therapeutic value for preventing heart attacks and strokes. A good example of these molecules is the disintegrins, which are competitive inhibitors of fibrinogen and interfere with platelet function. Disintegrins block cell-to-cell, cell-to-matrix interactions and signal transduction, thereby affecting the internal and external cellular activities of many different cells. It is our hypothesis that snake venoms are abundant and stable sources of many molecules and once purified, characterized, and cloned, could have many potential applications in medicine. Based on this hypothesis, the specific aims are to continue: (1) increasing the number of venomous snakes at the VRC for biomedical use; (2) characterizing snake venoms by high performance liquid chromatography and various assays to determine medical applications of venom molecules; (3) improving the Internet database for better dissemination of information to investigators; and (4) using the latest techniques such as proteomics to determine the molecular weights and amino acid sequences of venom proteins. The expected results will be that investigators will have access to a wide variety of properly maintained snakes, quality venoms and characterized fractions for biomedical research. The snakes, their venoms and venom fractions are placed on the NTRC Internet database, which can be queried. Venom can be ordered at a later date from the same snake, since all snakes have pit tags. Evaluation of the VRC will be based on the number of investigators using the venoms, publications, and information disseminated through the Internet database. The significance of the proposed research is that venom molecules have important biomedical applications in many different areas such as strokes, heart attacks, cancer and other medical problems.

描述（由申请人提供）：德克萨斯 A&M 大学金斯维尔分校的 Viper 资源中心 (VRC) P40 竞争性更新建议继续实现最初拨款申请的目标。资源将得到扩展，蛇毒、毒液成分、血液和细胞将提供给生物医学研究界。该提案的研究部分将侧重于通过表征毒液中发现的用于生物医学研究的重要分子来改进产品。由于大学的支持和 NIH 的资助，天然毒素研究中心 (NTRC) 内的 VRC 开发了配备最先进仪器的独特设施。世界上有 300 种不同种类的毒蛇，毒液是数千个分子的复杂混合物。许多毒蛇利用毒液干扰凝血级联来捕获猎物，从而导致动物失血过多而死亡。这些相同的分子可能具有预防心脏病和中风的治疗价值。这些分子的一个很好的例子是解整合素，它是纤维蛋白原的竞争性抑制剂并干扰血小板功能。解整合素阻断细胞与细胞、细胞与基质的相互作用和信号转导，从而影响许多不同细胞的内部和外部细胞活动。我们的假设是，蛇毒是许多分子的丰富且稳定的来源，一旦纯化、表征和克隆，可能在医学上有许多潜在的应用。基于这一假设，具体目标是继续：（1）增加 VRC 中用于生物医学用途的毒蛇数量； (2) 通过高效液相色谱和各种测定法表征蛇毒，以确定毒液分子的医学应用； （三）完善互联网数据库，更好地向调查人员传播信息； (4)利用蛋白质组学等最新技术测定毒液蛋白的分子量和氨基酸序列。预期的结果是，研究人员将能够获得各种妥善保存的蛇、优质毒液和用于生物医学研究的特征组分。这些蛇、它们的毒液和毒液成分都存放在NTRC互联网数据库中，可以查询。稍后可以从同一条蛇订购毒液，因为所有蛇都有坑标签。 VRC 的评估将基于使用毒液、出版物和通过互联网数据库传播的信息的调查人员数量。这项研究的意义在于，毒液分子在中风、心脏病、癌症和其他医学问题等许多不同领域具有重要的生物医学应用。

项目成果

A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain.

• DOI: 10.1038/nature10607
• 发表时间: 2011-11-16
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Bohlen, Christopher J.;Chesler, Alexander T.;Sharif-Naeini, Reza;Medzihradszky, Katalin F.;Zhou, Sharleen;King, David;Sanchez, Elda E.;Burlingame, Alma L.;Basbaum, Allan I.;Julius, David
• 通讯作者: Julius, David

Disintegrin, hemorrhagic, and proteolytic activities of Mohave rattlesnake, Crotalus scutulatus scutulatus venoms lacking Mojave toxin.

• DOI: 10.1016/j.cca.2005.04.001
• 发表时间: 2005-06
• 期刊: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
• 作者: E. Sánchez;J. Galan;Randy L. Powell;Steven R. Reyes;J. G. Soto;W. Russell;D. Russell;J. Pérez

Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell's viper venom-factor X activator (RVV-X).

elapidae，viperidae和纯化的Russell的Viper毒素毒液X激活剂（RVV-X）的28种蛇毒的抗凝剂和突发性特性的比较研究。

• DOI: 10.1016/j.toxicon.2010.05.012
• 发表时间: 2010-09-15
• 期刊: TOXICON
• 影响因子: 2.8
• 作者: Suntravat, Montamas;Nuchprayoon, Issarang;Perez, John C.
• 通讯作者: Perez, John C.

A novel method for the purification of low soluble recombinant C-type lectin proteins.

一种纯化低可溶性重组C型凝集素蛋白的新方法。

• DOI: 10.1016/j.bbrc.2012.07.128
• 发表时间: 2012
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Yin,Chunhui;Jia,Ying;Garcia,CarlosA
• 通讯作者: Garcia,CarlosA

Recombinant expression and affinity purification of snake venom gland parvalbumin in Escherichia coli.

蛇毒腺小白蛋白在大肠杆菌中的重组表达及亲和纯化。

• DOI: 10.1016/j.cbpa.2009.03.003
• 发表时间: 2009
• 期刊: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
• 作者: Jia,Ying;Perez,JohnC
• 通讯作者: Perez,JohnC