Phosphorelay signaling and regulation in bacteria

细菌中的磷中继信号传导和调节

• 批准号: 10573206
• 负责人: Mark D Goulian
• 金额: $ 39.63万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-10 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10573206
• 关键词: Anaerobiosis; Antibiotics; Bacteria; Bacterial Infections; Cell physiology; Critical Pathways; Development; Environment; Escherichia coli; Goals; Health; Infection; Maintenance; Nitrogen; Output; Oxygen; Phosphoric Monoester Hydrolases; Phosphotransferases; Physiology; Play; Potassium; Regulation; Research; Role; Signal Transduction; System; Treatment Protocols; extracellular; insight; microbiome; microbiota; novel; novel therapeutics; pathogen; phosphohistidine

Escherichia coli and related bacteria employ multi-step phosphorelays to sense intra- and extra-cellular environmental signals and to modulate diverse cellular processes. However, the significance of the multiple phosphoryl transfer steps that characterize these systems remains poorly understood. As part of our long- term goal to understand the interplay between the various signal transduction systems that bacteria employ to adapt to diverse environmental conditions, this proposal will explore several of these systems that are particularly tractable, due to well-established inputs and/or outputs, and that play critically important roles in E. coli physiology. The primary focus will be on two phosphorelays that are important for the transition to anaerobiosis, Arc and Tor, as well as a phosphohistidine phosphatase that modulates the nitrogen-related phosphotransferase system. Progress in understanding these networks will provide new insights into both the mechanisms enabling infections by pathogens and the maintenance of a healthy microbiota in host niches. The results may ultimately lead to the development of novel antibiotics or treatment regimens, as well as strategies for manipulating the microbiomes to maintain the health of the host.

大肠杆菌和相关细菌使用多步磷酸化继电器来感知细胞内和细胞外的 环境信号和调节不同的细胞过程。然而，多重性的重要性 表征这些系统的磷酰基转移步骤仍然知之甚少。作为我们长期- 术语的目的是了解细菌采用的各种信号转导系统之间的相互作用 为了适应不同的环境条件，本提案将探讨其中几种系统， 特别容易处理，由于良好的投入和/或产出，并发挥至关重要的作用， E.大肠杆菌生理学主要重点将放在两个磷继电器，这是重要的过渡到 厌氧，弧和Tor，以及磷酸组氨酸磷酸酶，调节氮相关的 磷酸转移酶系统了解这些网络的进展将为两者提供新的见解 使病原体感染和维持宿主健康微生物群的机制 壁龛这些结果可能最终导致开发新的抗生素或治疗方案， 以及操纵微生物组以维持宿主健康的策略。