New approaches for facilitating transcytosis of biologically active molecules

促进生物活性分子转胞吞作用的新方法

• 批准号: 10916060
• 负责人: Mark Henderson
• 金额: $ 12.58万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10916060
• 关键词: Antibodies; Automobile Driving; Basic Science; Blood - brain barrier anatomy; Brain; Chemistry; Encapsulated; Endothelium; Fc domain; Health; Human; Human Genome; Ligands; Methods; National Center for Advancing Translational Sciences; Phospholipids; Polysaccharides; Rare Diseases; Research; System; Translations; Vesicle; drug candidate; exosome; improved; novel strategies; pharmacologic; programs; tool; transcytosis

During this period, the collaborative team developed methods to produce lipidic vesicles mimicking the composition of exosomes. These vesicles were used to encapsulate small fluorescent molecules, and incorporation of functionalized phospholipids within these vesicles enabled the use of click chemistry. This approach will allow the attachment of ligands able to promote transcytosis across the blood-brain barrier. The team is currently validating methods to trim and modify the glycan of the Fc domain of antibodies to facilitate click chemistry, with the aim of clicking these antibodies to lipidic vesicles.

在此期间，合作团队开发了模拟外泌体组成的脂质囊泡的方法。这些囊泡被用来封装小的荧光分子，在这些囊泡中加入功能化的磷脂使点击化学的使用成为可能。这种方法将允许能够促进跨血脑屏障胞吞作用的配体的附着。该团队目前正在验证修剪和修改抗体Fc结构域聚糖的方法，以促进点击化学，目的是将这些抗体点击到脂质囊泡上。