Identification of small molecule degraders of XPB for inflammatory diseases

在此期间，该团队开发了一种基于细胞的高通量XPB表达筛查策略，并朝着实施1536孔版本的筛查进展。 这个合作团队的目标是在以前研究的基础上，通过识别小分子来复制螺内酯对XPB蛋白质稳定性的影响。XPB是一种核蛋白，调节炎性细胞因子的产生。早期翻译处(ETB)的总体目标是发现和开发新的小分子探针，并通过合作研究的模式催化早期开发过程。在每次合作中，NIH和校外疾病专家与ETB的早期发现团队合作，这些团队专门从事小分子筛查、药物化学和信息学。

During this period, the team has developed a strategy for a cell-based high-throughput screen of XPB expression and progressed towards implementing a 1536-well version of the assay for screening. This collaborative team aims to build on previous research by identifying small molecules that phenocopy the effect of spironolactone on XPB protein stability. XPB is a nuclear protein that regulates the production of inflammatory cytokines. The Early Translation Branch's (ETB) overall goal is to uncover and develop new small molecule probes and catalyze the process of early development through a model of collaborative research. With each collaboration, NIH and extramural disease experts work with the ETB's early discovery teams, who specialize in small molecule screening, medicinal chemistry and informatics.