Characterizing molecular interactomes using TurboID

使用 TurboID 表征分子相互作用组

• 批准号: 10916059
• 负责人: Mark Henderson
• 金额: $ 12.58万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10916059
• 关键词: Automobile Driving; Basic Science; Behavior; Biotin; Biotinylation; Dasatinib; Extramural Activities; Health; Human; Human Genome; Label; Ligase; Mass Spectrum Analysis; Methods; Methotrexate; Molecular; National Center for Advancing Translational Sciences; Proteins; Rare Diseases; Research; System; Translations; drug candidate; improved; pharmacologic; programs; small molecule; tool

During this period, the collaborative team has developed tools to explore new uses of TurboID as a proximity labeling tool to define small molecule interactomes. A variety of TurboID constructs and expression strategies were explored to characterize the behavior of the system. Several proof-of-concept small molecules, with known mechanisms of action, including dasatinib and methotrexate have been employed for these studies. The team has also pursued an optimized method for isolating biotinylated proteins for subsequent mass spectrometry and target identification.

在此期间，合作团队开发了工具，以探索TurboID作为邻近标记工具的新用途，以定义小分子相互作用组。 探索了各种TurboID构建体和表达策略来表征系统的行为。 几种具有已知作用机制的概念验证小分子，包括达沙替尼和甲氨蝶呤已用于这些研究。 该团队还寻求一种优化的方法来分离生物素化蛋白质，用于随后的质谱分析和目标识别。