LCM 220 LASER CAPTURE MICRODISSECTION SYSTEM

该提案要求为购买Pixcell II LCM 220激光捕获显微切割系统提供资金，该系统将安装在南卡罗来纳州大学医学院仪器资源设施（IRF）。IRF是一个核心研究仪器设施，每年有来自南加州大学医学院和附属南卡罗来纳州癌症中心、Dom VA和Richland纪念医院的150多名教职员工和学生使用。此外，来自南卡罗来纳州大学生物、化学、工程系和巴鲁克海洋研究所、克莱姆森大学、南卡罗来纳州医科大学、本尼迪克特学院和南卡罗来纳州周围几个行业的若干教员和学生目前也在使用综合资源联合会的设备。该提案的9个主要参与者代表了南加州大学医学院发育生物学和解剖学系、微生物学和免疫学系以及病理学系、南卡罗来纳州癌症中心和南加州大学生物学系的教师，他们共同获得了12项NIH研究赠款。为南卡罗来纳州哥伦比亚的南加州大学医学院IRF购买LCM 220将为南卡罗来纳州的几名研究人员提供对设备的集中访问。该文书的行政计划将以综合资源框架现有的行政计划为基础，该计划在过去几年中得到了成功的使用。对该系统的行政支持非常出色，承诺为LCM 220提供空间，为IRF技术人员提供协助系统运行的工资，并支付仪器年度服务合同的费用。激光捕获显微切割是在过去4-5年中开发的一种技术，其提供了从复杂组织切片中分离单个细胞或同质细胞群的方法，用于随后通过分子或生物化学技术进行分析。迄今为止，大多数应用都使用该系统从癌组织中分离特定细胞，用于随后的DNA、RNA和蛋白质分析。虽然本提案中的几个参与者将利用LCM 220来分析癌组织，但其他人提出将系统的使用扩展到分析从发育和患病的心脏和眼睛组织分离的组织。LCM技术在这些组织中的应用此前尚未报道。为了确保LCM是一种适当的技术，用于这些组织的拟议研究，我们还提出了初步的数据收集的协助下，大角星工程，制造商的Pixcell II LCM 220的技术人员。

This proposal requests funds for the purchase of the Pixcell II LCM 220 laser capture microdissection system that will be housed in the University of South Carolina School of Medicine Instrumentation Resource Facility (IRF). The IRF is a core research instrumentation facility utilized by more than 150 faculty staff and students from the USC School of Medicine and affiliated South Carolina Cancer Center, Dom VA and Richland Memorial Hospitals each year. In addition, equipment in the IRF is currently used by several faculty and students from the Departments of Biology, Chemistry, Engineering and the Baruch Marine Institute at the University of South Carolina, and Clemson University, the Medical University of South Carolina, Benedict College and several industries around the state of South Carolina. The 9 major participants on this proposal represent faculty from the Departments of Developmental Biology and Anatomy, Microbiology and Immunology, and Pathology in the USC School of Medicine, the South Carolina Cancer Center and the USC Department of Biology and collectively have 12 funded NIH research grants. Acquisition of the LCM 220 for the USC School of Medicine IRF in Columbia, SC will provide centralized access to the equipment for several research personnel in the State of South Carolina. The administrative plan for the instrument will be based on one that is already in place for the IRF which has been used successfully for the past several years. Administrative support for the system is excellent with a commitment to provide the space for the LCM 220, salaries for the IRF technical staff to assist with the operation of the system, and the cost of the annual service contract for the instrument. Laser capture microdissection is a technique that has been developed in the past 4-5 years that provides a method to isolate single cells or groups of homogeneous cells from a complex tissue section for subsequent analysis by molecular or biochemical techniques. The majority of applications to date have used the system to isolate specific cells from cancerous tissues for subsequent analysis of DNA, RNA and protein. While several of the participants in this proposal will utilize the LCM 220 for the analysis of cancerous tissues, others propose to extend the use of the system to analyze tissues isolated from developing and diseased heart and eye tissues. Application of the LCM technology to these tissues has not previously been reported. To insure that LCM is an appropriate technique for the proposed studies with these tissues we also present preliminary data collected with the assistance of the technical staff of Arcturus Engineering, manufacturers of the Pixcell II LCM 220.