A Laser Microbeam Biotechnology Resource

描述（由申请人提供）：激光微束和医学项目（LAMMP）是美国国立卫生研究院生物医学技术资源中心，致力于在生物学和医学中使用激光和光学。LAMMP位于贝克曼激光研究所（BLI）内，该研究所是加州大学欧文分校的跨学科生物医学研究、教学和临床设施。总体资源目标是促进中心在技术研究和发展、合作研究、服务、培训和传播方面的活动平衡。在LAMMP的第六次更新申请中，我们继续强调我们独特的能力，通过快速将基础科学和技术发现从“黑板到台式到床边”来促进“转化”研究。这是通过将最先进的光学技术与细胞和组织工程、组织病理学、临床前动物模型和临床护理的专业资源设施相结合来实现的。LAMMP提供显微镜和微束技术（MMT），用于高分辨率功能成像和活细胞和组织的操作，以及医学转化技术（MTT），用于非和微创监测，治疗和临床前动物模型和人类受试者的成像。此外，我们建议建立一个新的核心——虚拟光子技术（Virtual Photonics Technologies， VPT），用于开发计算模型和方法，以提高生物光子技术的性能，并提高光学测量的信息含量。提出了七个技术研究和开发项目，这些项目将导致制造几种新仪器以及创建多功能软件。这些项目建立在我们在光组织相互作用模型和生物光子学技术方面的长期专业知识基础上，包括激光微光束、非线性显微镜、光学相干断层扫描、空间/时间调制方法和漫射光学。LAMMP核心包含互补技术，能够定量表征、成像、扰动细胞和组织的结构和生化功能，具有可扩展的分辨率和深度灵敏度，范围从微米到厘米。建议进行合作研究，以推进这些技术，使其广泛可用，从而使生物学和医学方法成为可能。在整个拨款过程中，我们强调LAMMP技术与医学领域的相关性，如癌症、心血管疾病、代谢综合征和神经功能，以及基本的生物过程，如机械转导、伤口修复、血管生成、纤维化和细胞死亡。

DESCRIPTION (provided by applicant): The Laser Microbeam and Medical Program (LAMMP) is a NIH Biomedical Technology Resource Center dedicated to the use of lasers and optics in biology and medicine. LAMMP is located within the Beckman Laser Institute (BLI), an interdisciplinary biomedical research, teaching, and clinical facility at the University of California, Irvine. Overall resource objectives are to promote a well-balanced Center with activities in technological research and development, collaborative research, service, training, and dissemination. In this sixth renewal application of LAMMP, we continue to emphasize our unique capabilities to facilitate "translational" research by rapidly moving basic science and technology discoveries from "blackboard to benchtop to bedside". This is accomplished by combining state of the art optical technologies with specialized resource facilities for cell and tissue engineering, histopathology, pre-clinical animal models, and clinical care. LAMMP provides both Microscopy and Microbeam Technologies (MMT) for high-resolution functional imaging and manipulation of living cells and tissues and Medical Translational Technologies (MTT) for non- and minimally-invasive monitoring, treating, and imaging pre-clinical animal models and human subjects. In addition, we propose to establish a new core, Virtual Photonics Technologies (VPT) for developing computational models and methods that advance the performance of biophotonic technologies, and enhance the information content derived from optical measurements. Seven Technology Research and Development projects are proposed that will result in the fabrication of several new instruments as well as the creation of multi-functional software. These projects build on our longstanding expertise in light-tissue interaction models and Biophotonics technologies, including laser microbeams, non-linear microscopy, optical coherence tomography, spatial/temporal modulation methods, and diffuse optics. LAMMP cores contain complementary technologies that are capable of quantitatively characterizing, imaging, and perturbing structure and biochemical function in cells and tissues with scalable resolution and depth sensitivity ranging from micrometers to centimeters. Collaborative studies are proposed that advance these technologies so they become widely-available, enabling methods in Biology and Medicine. Throughout the grant we emphasize the relevance of LAMMP technologies to Medicine in areas such as cancer, cardiovascular disease, metabolic syndrome, and neurologic function, as well as fundamental biological process, such as mechano-transduction, wound repair, angiogenesis, fibrosis, and cell death.