Flexible, open source software for laser scanning microscopy

(在此处输入文本，它是的新摘要信息 你的申请表。此部分不得超过30行文本。) (C) 激光扫描显微镜(LSM)，包括共焦显微镜和双光子显微镜，在生物医学研究的许多领域中继续发挥着核心作用。仅在神经科学领域，申请数量就持续快速增长。然而，与目前在LSM硬件方面取得的进展(例如，更好、更便宜的光源、探测器和扫描仪)，使用LSM的一个主要限制仍然是商业软件的成本和缺乏灵活性。几年前，我们推出了Scanlmage，这是一个用于LSM的开源软件包。Scanlmage已经非常成功，现在被许多实验室使用，特别是用于双光子显微镜。然而，新类型的尖端应用需要新的功能。此外，将LSM与其他类型的数字或模拟刺激或采集(如电生理学)相结合通常很重要，这突显了对开源通用数据采集程序的相关、更基本的需求。在这里，我们建议(1)继续开发Scanlmage，重点放在活体成像上；(2)开发Ephus，这是一个新的补充程序，用于通用激光扫描和数据采集，如电生理记录和视频成像。这些程序大多是用MatLab编写的，具有底层的C主干。ScanImage的未来版本将支持新型扫描硬件；改进的感兴趣区域和相关扫描；快速z扫描；以及改进的在线多色显示。Ephus的未来版本将包括支持基于COD相机的图像采集和工具，以方便创建复杂的实验范例，例如基于激光扫描光刺激的神经电路映射，我们将维护ScanImage和Ephus的维基样式文档。我们相信，Scanlmage/Ephus将促进在众多实验室中进行令人兴奋的生物发现。 V0， (L) 0-0 CAA 、-、. (N� 。(D) 0(Q --� N-0

(Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text.) C.) Laser scanning microscopy (LSM), including confocal and 2-photon microscopy, continues to play a central role in many areas of biomedical research. In neuroscience alone, the number of applications continues to grow rapidly. However, in contrast to the ongoing advances made on the hardware aspects of LSM (eg. improved, cheaper light sources, detectors, and scanners), a major limitation to using LSM continues to be the cost and inflexibility of commercial software. Several years ago we introduced Scanlmage, an open source software package for LSM. Scanlmage has been highly successful and is now used by many labs, in particular for 2-photon microscopy. However, new types of cutting-edge applications require new functionality. In addition, it is usually important to combine LSM with other types of digital or analog stimulation or acquisition, such as electrophysiology, underscoring a related, more fundamental need, for an open-source general-purpose data acquisition program. Here, we propose (1) to continue to develop Scanlmage, with a focus on in vivo imaging and (2) to develop Ephus, a new and complementary program for general-purpose laser scanning and data acquisition, such as electrophysiological recording and video imaging. These programs are mostly written in Matlab, with an underlying C-backbone. Future releases of Scanimage will support new types of scanning hardware; improved region-of-interest and related scans; fast z-scanning; and, improved on-line multicolor display. Future releases of Ephus will include support for COD camera -based image acquisition and tools to facilitate easy creation of complex experimental paradigms, such as laser scanning photostimulation based mapping of neural circuits, We will maintain wiki-style documentation for Scanimage and Ephus. We believe that Scanlmage/Ephus will facilitate exciting biological discovery in numerous laboratories. v0, (l) 0-0 CAA ,-,. (n� .(D 0(Q --� N-0