Aromatic compound degradation: characterizing the benzoate oxidation (BOX) pathway in Burkholderia xenovorans LB400

芳香族化合物降解：表征异源伯克霍尔德杆菌 LB400 中的苯甲酸酯氧化 (BOX) 途径

• 批准号: 344815-2007
• 负责人: Boulanger, Martin
• 金额: $ 3.24万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326035
• 关键词: Aromatic; compound; degradation; characterizing; benzoate

Aromatic compounds compose approximately 25% of the Earth's biomass and are the second most abundant class of natural products next to carbohydrates. Due to the inherent stability of the aromatic ring, natural attenuation of these compounds is very slow. Many bacteria, however, have evolved biodegradative capabilities that can make use of a wide variety of aromatic compounds as sources of energy. These capabilities have evolved due to widespread availability of many aromatic compounds in the environment in the form of recycled plant material. Man-made aromatic pollutants, however, pose major challenges for bacterial degradation. Not only are these compounds more chemically complex, but they have only been exposed to microbial degraders for a relatively short period of time. As a result, these xenobiotic compounds, many of which are derived from crude oil, persist in the environment and cause irreversible damage to the biosphere. The most effective strategy to stimulate the removal of these contaminants from the environment is to manipulate the metabolic pathways of bacteria to increase protein stability and broaden substrate specificity. As a prerequisite step to the rationale engineering of these biological systems, detailed descriptions of the enzymes involved are essential. To this end, the goal of this research program is to define the mechanism by which aromatic compounds are degraded via the novel benzoate oxidation (box) pathways in B. xenovorans LB400. The long-term goal of this research is to design more efficient strategies for the accelerated bioremediation of environmentally toxic aromatic based pollutants.

芳香族化合物占地球生物量的约25%，是仅次于碳水化合物的第二大类天然产物。由于芳香环的固有稳定性，这些化合物的自然衰减非常缓慢。然而，许多细菌已经进化出生物降解能力，可以利用各种芳香族化合物作为能量来源。由于许多芳香族化合物以回收植物材料的形式在环境中广泛可用，这些能力已经发展。然而，人造芳香族污染物对细菌降解构成了重大挑战。这些化合物不仅在化学上更复杂，而且它们只暴露于微生物降解剂相对较短的时间。因此，这些异生素化合物（其中许多来自原油）在环境中持续存在，对生物圈造成不可逆转的损害。刺激从环境中去除这些污染物的最有效策略是操纵细菌的代谢途径以增加蛋白质稳定性并拓宽底物特异性。作为这些生物系统的基本原理工程的先决条件，所涉及的酶的详细描述是必不可少的。为此，本研究计划的目标是确定芳香族化合物通过B中的新型苯甲酸酯氧化（box）途径降解的机制。xenovorans LB 400.本研究的长期目标是设计更有效的策略，加速对环境有毒的芳香族污染物的生物修复。