Gradient thermal cycler

梯度热循环仪

• 批准号: 359503-2008
• 负责人: Cristescu, Melania
• 金额: $ 1.32万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364390
• 关键词: Gradient; thermal; cycler

SummaryThe requested equipment (the Eppendorf Mastercycler ep Gradient Thermal Cycler) will support diverse research projects which can be categorized into three main areas: genomics, population genetics and conservation.1) Population genetics:  The requested equipment will be used for studying the role of recombination in the long term evolution of sexual and asexual lineages. It will provide high through-put DNA amplification capacity necessary for screening the genome-wide genetic polymorphism in sexual and asexual Daphnia lineages.  2) Genomics:  Comparative linkage mapping can provide unexpected insights into chromosome evolution, genome reorganization and speciation and can reveal the nature and scale of recombination rate variation across genomes.  The requested equipment will be used for investigating recombination rate variation among closely related species of Daphnia.3) Conservation:  The application of molecular approach to conservation biology has revolutionized the management of endangered species and ecosystems. The requested equipment will play a key role in the identification of routes of aquatic species invasions (e.g. ctenophore Mnemiopsis, plus five species of tunicates).This equipment will support existing research programs and timely completion of graduate research.

概述所申请的设备（Eppendorf Mastercycler ep Gradient Thermal Cycler）将支持各种研究项目，这些项目可分为三个主要领域：基因组学、种群遗传学和保护。1）种群遗传学：所申请的设备将用于研究重组在有性和无性谱系长期进化中的作用。这将提供高通量的DNA扩增能力，用于筛选有性和无性生殖水蚤谱系的全基因组遗传多态性。比较连锁图谱可以为染色体进化提供意想不到的见解，基因组重组和物种形成，可以揭示基因组间重组率变异的性质和规模。所要求的设备将用于调查重组率变异3）保护：分子生物学方法在保护生物学中的应用彻底改变了濒危物种和生态系统的管理。所要求的设备将在查明水生物种入侵途径方面发挥关键作用（例如栉水母拟记忆虫和五种被囊动物），这些设备将支持现有的研究方案和及时完成研究生研究。