Solid state NMR spectroscopy: chemical and biological applications

固态核磁共振波谱：化学和生物应用

• 批准号: 203308-2006
• 负责人: Wu, Gang
• 金额: $ 4.47万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=392790
• 关键词: Solid; state; NMR; spectroscopy; chemical

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique for studying molecular structure, dynamics and chemical bonding. To date most successful NMR applications have focused on detection of atomic nuclei with a nuclear spin quantum number of ½. However, over 70% of the elements in the Periodic Table have isotopes whose nuclear spin quantum number is greater than ½ such as Na-23 (spin-3/2), K-39 (spin-3/2) and O-17 (spin-5/2). These nuclei are known as quadrupolar nuclei. Because quadrupolar nuclei possess unfavorable nuclear properties, many of them have often been considered "NMR invisible". The primary objective of our research program is to develop new NMR techniques to make quadrupolar nuclei accessible and, furthermore, to apply these techniques to biomolecular systems such as DNA and ion channel proteins. NMR for quadrupolar nuclei has remained one of the most challenging problems to experimentalists in the field of NMR research. Our proposed research may provide new possibilities in two general areas: (1) direct detection of alkali metal ions in DNA and ion channel proteins and (2) development of O-17 NMR as a new probe to biological structures. These represent new research directions with considerable potential. Successful applications will shed new light into several unsolved problems in chemistry and biochemistry. The proposed research will also have a significant impact on the general development of NMR spectroscopy. The students trained in this research program will learn important knowledge about preparation of DNA and protein samples and application of advanced biophysical characterization methods, as well as critical thinking skills to be successul in either academic or industrial environment.

核磁共振波谱是研究分子结构、动力学和化学键的一种强有力的分析技术。迄今为止，大多数成功的核磁共振应用都集中在检测核自旋量子数为1 / 2的原子核上。然而，元素周期表中超过70%的元素的原子核自旋量子数大于1 /2的同位素，如Na-23（自旋3/2）、K-39（自旋3/2）和O-17（自旋5/2）。这些原子核被称为四极核。由于四极核具有不利的核性质，许多四极核常被认为是“核磁共振不可见”的。我们研究项目的主要目标是开发新的核磁共振技术，使四极核易于获取，并且将这些技术应用于生物分子系统，如DNA和离子通道蛋白。四极核的核磁共振一直是核磁共振研究领域中最具挑战性的问题之一。我们提出的研究可能在两个一般领域提供新的可能性：(1)直接检测DNA和离子通道蛋白中的碱金属离子；(2)开发O-17核磁共振作为生物结构的新探针。这些都代表了具有相当潜力的新研究方向。成功的应用将为化学和生物化学中一些尚未解决的问题提供新的思路。所提出的研究也将对核磁共振波谱学的整体发展产生重大影响。在这个研究项目中训练的学生将学习DNA和蛋白质样品的制备和先进的生物物理表征方法的应用的重要知识，以及在学术或工业环境中取得成功的批判性思维技能。