Roles of virus-integrin interactions and rotavirus modulation of host cell responses in viral pathogenesis

病毒-整合素相互作用和轮状病毒对宿主细胞反应的调节在病毒发病机制中的作用

• 批准号: nhmrc : 350252
• 负责人: A/Pr Barbara Coulson
• 金额: $ 31.61万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2005
• 资助国家: 澳大利亚
• 起止时间: 2005-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/roles-virus-integrin-viral-pathogenesis/96379
• 关键词: Roles; virus; integrin; interactions; rotavirus

Rotaviruses are the main cause of severe gastroenteritis in children, and cause 1 in 27 Australian children under the age of 5 years to spend time in hospital. There is currently no rotavirus vaccine available. We aim to discover how rotavirus interacts with host cells. This information is necessary to formulate a safe and effective vaccine, or a therapeutic agent that can block virus growth in host cells. Previously, we showed that rotavirus attaches to cells and enters them using several members of the integrin protein family that are present on the surface of the cells. Integrins are critical for cell adhesion, survival and communication. In this project, we will identify how rotavirus usage of integrins modulates cell functions. This will help us understand how rotavirus causes disease, how virus spreads in the body and how the immune response defends us from rotavirus. Rotavirus binds integrins using particular stretches of protein sequence that we have shown are also present in other human viral pathogens that cause hepatitis, AIDS and measles. We will determine if these other viruses also recognize integrins.

轮状病毒是儿童严重胃肠炎的主要原因，导致27名5岁以下澳大利亚儿童中有1人住院。目前还没有轮状病毒疫苗。我们的目标是发现轮状病毒如何与宿主细胞相互作用。这些信息对于制定安全有效的疫苗或可以阻断病毒在宿主细胞中生长的治疗剂是必要的。以前，我们表明轮状病毒附着在细胞上，并使用存在于细胞表面的整合素蛋白家族的几个成员进入细胞。整合素对细胞粘附、存活和通讯至关重要。在这个项目中，我们将确定轮状病毒如何利用整合素调节细胞功能。这将帮助我们了解轮状病毒如何引起疾病，病毒如何在体内传播以及免疫反应如何保护我们免受轮状病毒的侵害。轮状病毒利用特定的蛋白质序列片段结合整合素，我们已经证明这些蛋白质序列片段也存在于引起肝炎、艾滋病和麻疹的其他人类病毒病原体中。我们将确定这些其他病毒是否也识别整合素。