Targeting Protein Synthesis in the Apicoplast and Cytoplasm of Plasmodium

疟原虫顶质体和细胞质中的靶向蛋白质合成

• 批准号: nhmrc : 520700
• 负责人: A/Pr Stuart Ralph
• 金额: $ 30.26万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Strategic Awards
• 财政年份: 2010
• 资助国家: 澳大利亚
• 起止时间: 2010-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/targeting-protein-synthesis-cytoplasm-plasmodium/94811
• 关键词: Targeting; Protein; Synthesis; Apicoplast; Cytoplasm

New antimalarial drugs are desperately needed. Protein synthesis in Plasmodium falciparum is a validated target for existing drugs and is a promising target for new drugs. This project brings together malaria biologists with chemists and computer scientists to explore this promising field. We will apply modern methods of drug target characterisation to find the most promising enzyme targets involved in protein synthesis and to identify inhibitors as leads for developing antimalarial therapies. Australian researchers involved in this project will provide expertise in bioinformatic prioritisation of Plasmodium drug targets from the aminoacyl tRNA synthetase family of enzymes. We will use structural modelling and docking experiments to identify promising antimalarial inhibitors, and will optimise assays to assess the effects of these inhibitors. We will also apply modern molecular biology tools to validate these enzymes as anti-malarial drug targets.

迫切需要新的抗疟疾药物。恶性疟原虫中的蛋白质合成是现有药物的验证靶点，并且是新药的有希望靶点。该项目将疟疾生物学家、化学家和计算机科学家聚集在一起，探索这一有前途的领域。我们将应用药物靶点表征的现代方法来寻找参与蛋白质合成的最有前途的酶靶点，并确定抑制剂作为开发抗疟疗法的先导。参与该项目的澳大利亚研究人员将提供从氨酰tRNA合成酶家族中对疟原虫药物靶标进行生物信息学优先排序的专业知识。我们将使用结构建模和对接实验来确定有前途的抗疟抑制剂，并将优化分析，以评估这些抑制剂的效果。我们还将应用现代分子生物学工具来验证这些酶作为抗疟疾药物靶标。