Zebrafish housing system for mutants and transgenics

用于突变体和转基因的斑马鱼饲养系统

• 批准号: 389994-2010
• 负责人: Waskiewicz, Andrew
• 金额: $ 5.57万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443080
• 关键词: Zebrafish; housing; system; mutants; transgenics

Zebrafish research has undergone two major technological revolutions in the last six years. These two developments, transposase-mediated transgenesis and targeted gene knockouts, have greatly facilitated the creation of transgenic and mutant strains, respectively. This has made zebrafish into an extremely powerful model vertebrate. Researchers in the eight applicant laboratories have been unable to fully utilize these new technologies owing to the inadequacy of current zebrafish housing. This proposal is for the acquisition of a new zebrafish facilty that will be used to maintain this increasing diversity of transgenic and mutant lines. This four rack system will include filtration and sterilization components as well as monitoring systems that continuously evaluate and adjust pH and conductivity. This system will house approximately 11,000 breeding adults and be used by eight independent laboratories at the University of Alberta. With >50 HQP in the applicant laboratories devoted to zebrafish research, this proposed equipment installation will have an extremely broad and powerful impact on the quality of training and resultant research findings.

在过去的六年里，斑马鱼研究经历了两次重大的技术革命。转座酶介导的转基因和靶向基因敲除这两项进展，分别极大地促进了转基因菌株和突变菌株的产生。这使得斑马鱼成为一种极其强大的脊椎动物模型。由于目前斑马鱼住房不足，八个申请实验室的研究人员无法充分利用这些新技术。这项提案是为了获得一个新的斑马鱼设施，将用于维持这种日益增加的转基因和突变系的多样性。这四个机架系统将包括过滤和灭菌组件以及监测系统，不断评估和调整pH值和电导率。该系统将容纳大约11000只繁殖成虫，由阿尔伯塔大学的8个独立实验室使用。由于申请实验室中有bbbb50 HQP专门用于斑马鱼研究，该提议的设备安装将对培训质量和最终研究结果产生极其广泛和强大的影响。