Zebrafish housing system for mutants and transgenics

在过去的六年里，斑马鱼研究经历了两次重大的技术革命。转座酶介导的转基因和靶向基因敲除这两个发展分别极大地促进了转基因和突变株的创建。这使得斑马鱼成为一种非常强大的模式脊椎动物。由于目前斑马鱼饲养条件不足，八个申请实验室的研究人员无法充分利用这些新技术。这项提议是为了获得一个新的斑马鱼设施，将用于保持这种不断增加的转基因和突变系的多样性。这四个机架系统将包括过滤和灭菌组件以及持续评估和调整pH值和电导率的监测系统。该系统将容纳大约11，000只繁殖成虫，并由阿尔伯塔大学的八个独立实验室使用。由于申请实验室中有超过50名HQP致力于斑马鱼研究，因此拟议的设备安装将对培训质量和最终的研究成果产生极其广泛和强大的影响。

Zebrafish research has undergone two major technological revolutions in the last six years. These two developments, transposase-mediated transgenesis and targeted gene knockouts, have greatly facilitated the creation of transgenic and mutant strains, respectively. This has made zebrafish into an extremely powerful model vertebrate. Researchers in the eight applicant laboratories have been unable to fully utilize these new technologies owing to the inadequacy of current zebrafish housing. This proposal is for the acquisition of a new zebrafish facilty that will be used to maintain this increasing diversity of transgenic and mutant lines. This four rack system will include filtration and sterilization components as well as monitoring systems that continuously evaluate and adjust pH and conductivity. This system will house approximately 11,000 breeding adults and be used by eight independent laboratories at the University of Alberta. With >50 HQP in the applicant laboratories devoted to zebrafish research, this proposed equipment installation will have an extremely broad and powerful impact on the quality of training and resultant research findings.