Zebrafish housing system and ultrafreezer

斑马鱼饲养系统和超冷冻箱

• 批准号: 405930-2011
• 负责人: Vijayan, Mathilakath
• 金额: $ 2.13万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472342
• 关键词: Zebrafish; housing; system; ultrafreezer

The objective of this application is to obtain a zebrafish housing system and ultrafreezer for short-term and long-term maintenance of zebrafish, and storage of their tissues for molecular research. The zebrafish housing system will consist of racks that can accommodate different size tanks for growing embryos, juveniles and for breeding adults. This set-up will have a small footprint but can hold several tanks for exposure studies pertaining to elucidation of glucocorticoid receptor signaling pathways involved in stress adaptation. The ultrafreezer will be critical for the storage of tissue samples, including brain, gill, muscle, heart, liver, kidney, embryos and whole organism for gene and protein expression studies. This equipment is essential for my research program due to the added numbers of research personnel in my laboratory. The lack of a zebrafish housing system and ultrafreezer space to accommodate their demands is impacting my research program. The zebrafish housing system and ultrafreezer will be available to researchers at the University of Waterloo.

本申请的目的是获得一种用于斑马鱼短期和长期维护的斑马鱼圈养系统和超冷冻机，以及用于分子研究的斑马鱼组织的储存。斑马鱼的饲养系统将由机架组成，可以容纳不同大小的水箱，用于生长胚胎，幼鱼和繁殖成年鱼。该装置占地面积小，但可以容纳几个罐，用于与阐明应激适应中涉及的糖皮质激素受体信号通路有关的暴露研究。超低温冷冻机对于组织样品的储存至关重要，包括脑、鳃、肌肉、心脏、肝脏、肾脏、胚胎和用于基因和蛋白质表达研究的整个生物体。由于实验室研究人员的增加，这些设备对我的研究计划至关重要。缺乏一个斑马鱼住房系统和超冷冻空间，以满足他们的需求是影响我的研究计划。斑马鱼住房系统和超冷冻室将提供给滑铁卢大学的研究人员。