Zebrafish housing system and ultrafreezer

本申请的目的是获得一个斑马鱼住房系统和超冷冻机，用于短期和长期维护斑马鱼，并储存其组织用于分子研究。斑马鱼的住房系统将由架子组成，可以容纳不同大小的水箱，用于生长的胚胎、幼鱼和繁殖的成年鱼。该装置占地面积小，但可以容纳几个容器，用于与阐明应激适应中涉及的糖皮质激素受体信号通路有关的暴露研究。超冷冻机对于组织样本的储存至关重要，包括脑、鳃、肌肉、心脏、肝脏、肾脏、胚胎和用于基因和蛋白质表达研究的整个生物体。由于我的实验室增加了研究人员，这台设备对我的研究计划是必不可少的。缺乏斑马鱼的住房系统和超冷冻机空间来满足它们的需求，这影响了我的研究计划。滑铁卢大学的研究人员将获得斑马鱼住房系统和超冷冻机。

The objective of this application is to obtain a zebrafish housing system and ultrafreezer for short-term and long-term maintenance of zebrafish, and storage of their tissues for molecular research. The zebrafish housing system will consist of racks that can accommodate different size tanks for growing embryos, juveniles and for breeding adults. This set-up will have a small footprint but can hold several tanks for exposure studies pertaining to elucidation of glucocorticoid receptor signaling pathways involved in stress adaptation. The ultrafreezer will be critical for the storage of tissue samples, including brain, gill, muscle, heart, liver, kidney, embryos and whole organism for gene and protein expression studies. This equipment is essential for my research program due to the added numbers of research personnel in my laboratory. The lack of a zebrafish housing system and ultrafreezer space to accommodate their demands is impacting my research program. The zebrafish housing system and ultrafreezer will be available to researchers at the University of Waterloo.