Nanopore analysis of peptides and proteins

肽和蛋白质的纳米孔分析

• 批准号: 203084-2008
• 负责人: Lee, Jeremy
• 金额: $ 2.5万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=453114
• 关键词: Nanopore; analysis; peptides; proteins

Nanopore analysis enables the investigation of single molecular events. A single pore (either of bacterial origin or solid state) is inserted in a membrane and isolated between two chambers. When a voltage is applied a constant current will flow through the pore. If a molecule bumps into the pore or translocates through the pore a blockade current (I) will be observed for a particular time (T). Structural and sequence information is embedded in the parameters I and T. More recently, some preliminary investigations of the translocation of peptides (alpha-helices) and proteins (HPr, 86 amino acids) have been completed in Saskatoon. Here I propose to extend these studies to investigate:

纳米孔分析使单分子事件的研究成为可能。将单孔(细菌来源或固体)插入膜中，并将其隔离在两个腔室之间。当施加电压时，会有恒定电流流过气孔。如果一个分子撞击到孔中或通过孔转移，将在特定时间(T)内观察到阻塞电流(I)。结构和序列信息嵌入在参数I和T中。最近，在萨斯卡通完成了对多肽(α-螺旋)和蛋白质(HPR，86个氨基酸)易位的一些初步研究。在此，我建议扩大这些研究的范围，以调查：