Nanopore analysis of peptides and proteins

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Nanopore analysis enables the investigation of single molecular events. A single pore (either of bacterial origin or solid state) is inserted in a membrane and isolated between two chambers. When a voltage is applied a constant current will flow through the pore. If a molecule bumps into the pore or translocates through the pore a blockade current (I) will be observed for a particular time (T). Structural and sequence information is embedded in the parameters I and T. More recently, some preliminary investigations of the translocation of peptides (alpha-helices) and proteins (HPr, 86 amino acids) have been completed in Saskatoon. Here I propose to extend these studies to investigate: