Anti-biofilm activity of biophotonic gels

生物光子荧光凝胶有可能彻底改变细菌感染的治疗， 控制细菌生物膜的生长。在我们与Klox正在进行的Engage合作中， 蒙特利尔大学的工作，涉及不同的凝胶的物理化学含量和结构 在一些实施方案中，本发明的组合物与它们对变形链球菌（Streptococcus mutans）（革兰氏阳性细菌，通常在大肠杆菌中发现）的生物膜的作用相关。 牙齿）。这项工作已经能够证明，由Klow生产的生物光子凝胶在 生物膜。此外，该项目还确定了凝胶成分，这些成分似乎是 控制生物膜内的细菌生长。因此，人们对改变以下物质的浓度非常感兴趣： 水凝胶中的每种活性组分，以优化进入生物膜的扩散性和 凝胶的总体抗菌效果。此外，生物光子卡波姆凝胶的抗菌活性 并且它们与生物膜相互作用的机制尚未被研究。因此，该项目将 评价具有不同浓度的过氧化脲（0、3、9和12%）的凝胶的抗菌活性。 以及凝胶中三种活性荧光团的不同浓度。由于凝胶的结构不同， 根据它们的制备方法，不同的光诱导持续时间（1、3、5分钟或更长，如果 也将进行调查。该研究将有助于深入了解凝胶的相互作用机制 与生物膜，这将使他们的优化基于健全的科学。

Biophotonic, fluorescent gels have the potential to revolutionize the treatment of bacterial infections and control the growth of bacterial biofilms. In our ongoing Engage collaboration with Klox, the team at the University of Montreal has worked to relate the physicochemical content and structure of the different gel formulations to their effects on a biofilm of Streptococcus mutans (gram positive bacteria commonly found on the teeth). This work has been able to demonstrate that the biophotonic gels produced by Klow work well on the biofilms. Furthermore, the project has identified the gel components that appear to be key with respect to controlling bacterial growth within the biofilms. There is thus great interest in varying the concentrations of each of the active components in the hydrogels in order to optimize both diffusivity into the biofilms and the overall antibacterial effects of the gels. Furthermore, the antibacterial activity of the biophotonic carbopol gels and their mechanism of interaction with the biofilms has not yet been investigated. Therefore, this project will evaluate the antibacterial activity of the gels with different concentrations of urea peroxide (0, 3, 9 and 12%) and different concentrations of the three active fluorophores in the gels. Since the structure of the gels varies according to their method of preparation, different durations of photo-induction (1, 3, 5 minutes or longer, if required) will also be investigated. The study will provide insight into the mechanism of interaction of the gels with the biofilms, which will enable their optimization based upon sound science.