Optimization of IgY and phospholipid extraction

多克隆免疫球蛋白Y（IgY）抗体是鸡体内主要的一类抗体。被动式口语 在哺乳动物中施用IgY抗体通过预防胃肠道感染来对抗特异性胃肠道感染。 病原微生物对肠道的粘附。鸡卵是IgY的丰富来源， 从蛋中分离IgY比从鸡的血液中分离的常规抗体具有许多优点， 动物优点是：（1）收集卵子是非侵入性的，而不是使动物流血， (2)在整个产蛋周期中可反复产生大量IgY，（3）免疫力较强 对哺乳动物抗原的反应是由于哺乳动物之间的进化遗传距离而实现的。 哺乳动物和鸟类。用于从蛋中提取IgY的典型方法包括有机-水相萃取法和有机-水相萃取法。 IgY的提取、沉淀、超浓缩、超滤、亲和层析及其组合 它们的绝大多数IgY的提取方法依赖于有机溶剂和相关试剂的使用 其不是生物相容的并且限制了用于人类目的的应用。替代IgY分离方法 或者由于蛋白质变性而遭受低的提取产率，或者难以规模化。 AXXARYS公司，一家专门生产用于治疗糖尿病的活性成分的公司， 目的是开发和优化从蛋黄中连续提取IgY， 生物相容性水两相系统（ATPS）用于萃取和超临界流体萃取（SFE）。ATPs 或ATPE（含水两相萃取）是一种液-液萃取方法，其涉及分配 通过原位添加诸如聚合物的物质将水溶液分成两个不混溶的相， 将盐添加到水溶液中。ATPE/ATPS是一种众所周知的温和的分离方法， 纯化蛋白质和抗体。该方法的优点包括高容量，相体积 还原、快速分离、易于放大。

Polyclonal immunoglobulin Y, IgY, antibodies are the major class of antibodies in chickens. The passive oral administration of IgY antibodies in mammals combat specific gastrointestinal infections by preventing the adhesion of pathogenic micro-organisms to the intestines. Eggs from chickens are a rich source of IgY and the isolation of IgY from eggs have many advantages over conventional antibodies isolated from the blood of animals. The advantages are that: (1) the collection of eggs is non-invasive as opposed to bleeding the animal, (2) large amounts of IgY can be produced repeatedly through the egg laying cycle, and (3) a stronger immune response against mammalian antigens is achieved as a result of the evolutionary phylogenic distance between mammals and birds. Typical methods employed for the extraction of IgY from eggs include organic-aqueous extraction, precipitation of IgY, ultracentrifugation, ultrafiltraton, affinity chromatography and combinations thereof. The vast majority of IgY extraction methods rely on the use of organic solvents and related reagents which are not biocompatible and limit the application for human purposes. Alternative IgY isolation methods either suffer from low extraction yields because of protein denaturation or are difficult to scale. The present proposal by AXXARYS Corp, a company specialized in the production of active ingredients for nutraceuticals, aims to develop and optimize the sequential extraction of IgY from egg yolks using biocompatible aqueous two phase system (ATPS) for extraction and SFE (supercritical fluid extraction). ATPS or ATPE (aqueous two-phase extraction) is a liquid-liquid extraction method which involves the partitioning of the aqueous solution into two immiscible phases by the in-situ addition of substances such as polymers and salts to the aqueous solution. The ATPE/ATPS is a well-known gentle method for the separation and purification of proteins and antibodies. The advantages of the method include high capacity, phase volume reduction, rapid separation and easy scale-up.