Enhancing quality assurance of medical marijuana using DNA fingerprinting

使用 DNA 指纹识别增强医用大麻的质量保证

• 批准号: 485869-2015
• 负责人: Punja, Zamir
• 金额: $ 0.91万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Engage Plus Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585244
• 关键词: Enhancing; quality; assurance; medical; marijuana

Marijuana (Cannabis sativa L.) is increasingly being recognized for its properties in treating a number of human diseases, such as multiple sclerosis, chronic pain, seizures and PTSD. Marijuana plants may be initiated from seeds but more commonly are propagated from vegetative cuttings originating from stock plants (mother plants). Seeds are used when a new genetic line (strain) is being considered, but is subject to variation in phenotypic characteristics in the offspring due to segregation. It is not uncommon for a potential mix-up of genetic lines to occur during commercial production, or for seeds or vegetative propagation to give rise to unusual phenotypes. This creates a problem for quality assurance as well as for monitoring a particular line for genetic stability over time. The availability of a DNA fingerprinting method that can be used to distinguish among selected lines, as well as to study the extent of genetic variation among a wide range of lines and seed sources, would be invaluable as a rapid screen for quality assurance and genetic fidelity. In the preceding Engage grant, a set of 7 ISSR primers was identified that produced distinct banding patterns when used with a broad range of DNA sources of Cannabis. This primer set showed that a significant level of genetic variation exists amongst 40 strains of medical marijuana, something not previously reported. An additional set of primers (SFU1, SFU2) was developed that could distinguish male from female plants of marijuana. The objectives of this research are to: 1) Determine the variables affecting the utility of a plant sex testing kit based on primers SFU1-SFU2 developed in the previous work; 2) Continue to evaluate the set of 7 ISSR primers for suitability to develop a DNA fingerprinting test kit that could be utilized commercially to identify select marijuana strains; 3) Assess the conditions that can influence the variability of results in objectives (1) and (2) above; 4) Conduct DNA sequencing to verify the relationships among the different marijuana strains, as well as between female and male plants, and compare the results to the Cannabis genome sequence available on-line and Genbank sequences.

大麻（Cannabis sativa L.）越来越多地被认识到其在治疗一些 人类疾病，如多发性硬化症，慢性疼痛，癫痫发作和创伤后应激障碍。海洋植物可以开始 从种子繁殖，但更常见的是从源自原种植物（母本）的营养扦插繁殖 植物）。当考虑一个新的遗传品系（品系）时，使用种子，但种子的遗传特性可能会发生变化。 由于分离，后代的表型特征。这是不寻常的一个潜在的混淆， 在商业生产过程中出现的遗传系，或用于种子或无性繁殖以产生 不寻常的表型这对于质量保证以及对于监控特定生产线产生了问题， 随着时间的推移，遗传稳定性。DNA指纹分析方法的可用性，可用于区分 在选定的品系之间，以及研究广泛的品系和种子之间的遗传变异程度 来源，将是宝贵的，作为一个快速筛选质量保证和遗传保真度。前项 Engage grant，鉴定了一组7个ISSR引物，当与一个引物一起使用时，它们产生不同的带型。 大麻的DNA来源广泛。该引物组表明，一个显着水平的遗传变异， 存在于40种医用大麻中，这是以前没有报道过的。附加的一组 本研究开发了一对能区分大麻雌雄株的引物（SFU 1、SFU 2）。的 本研究的目的是：1）确定影响植物性别检测试剂盒效用的变量， 2）继续对7对引物进行扩增， 开发DNA指纹检测试剂盒的适用性，该试剂盒可用于商业鉴定选择 3）评估可能影响目标（1）和（2）中结果的可变性的条件 4）进行DNA测序，以验证不同大麻品种之间的关系，以及 在雌性和雄性植物之间，并将结果与在线提供的大麻基因组序列进行比较 和基因库序列。