High Resolution ESI Mass Spectrometer

我们申请资金购买一台高分辨率电喷雾电离质谱仪（HR-ESI-MS）。这台HR-ESI-MS将在UBC化学质谱分析设备中进行操作和维护，在那里它将取代一台19年的摇摇晃晃的仪器。这台之前的仪器在2017年因为维修已经有4个月无法使用了，很多替换零件已经没有了。一次灾难性的故障使仪器无法再修复只是时间问题。这台仪器的丢失将影响20个研究小组和150多个HQP的研究进展，使我们整个部门的进展停滞不前，包括温哥华和奥肯那根校区，并影响到TRIUMF和其他地方的研究小组。新仪器将配备(1)ESI离子源，这是一种理想的极性有机分子和金属配合物的“软”电离技术；(2)飞行时间质谱仪，可为100 - 10,000 Da的化合物提供高质量分辨率（bbb20 000）和高质量精度（<1 ppm）；(3)一台高效液相色谱仪，使混合物能够分离，并允许经过培训的HQP自助服务；(4)先进的软件，可快速测定分子式。该仪器将用于我们部门的实验，包括对合成和生化研究中衍生的极性有机化合物和金属配合物的元素组成进行精确的质量测量；天然产物的结构解析；中等大小分子的分子量测定；以及反应混合物的分析。由于这些实验对整个部门的研究小组至关重要，UBC化学已将此次采购视为其最优先的设备采购，并将提供超出NSERC RTI计划要求的150,000美元的大量资金。支持该申请的申请人和共同申请人是Raymond Andersen（天然产物分离），Marco Ciufolini（合成化学），Gregory Dake（合成化学），Frederic Menard （UBC-Okanagan，合成化学和神经生物学），Christopher Orvig（药物无机化学），Katherine Ryan（生物合成），Paul Schaffer （TRIUMF，放射性示踪成像）和Stephen Withers（糖化学和生物学）。在NSERC Discovery， NSERC Create， NSERC- cihr CHRP和其他资助的支持下，我们目前在我们的个人小组中总共培训了bb80名学员。购买这台仪器对我们研究计划的成功至关重要。

We request funds to purchase a high resolution electrospray ionization mass spectrometer (HR-ESI-MS). This HR-ESI-MS will be operated and maintained in the UBC Chemistry Mass Spectrometry Facility, where it will replace a 19 year old, faltering instrument. This previous instrument has been unusable for 4 months in 2017 due to repairs, and many replacement parts are no longer available. It is only a matter of time before one catastrophic failure renders the instrument no longer repairable. The loss of this instrument would affect the research progress of 20 research groups and over 150 HQP, stalling progress across our entire department, including both the Vancouver and Okanagan campuses, and affecting research groups at TRIUMF and beyond. The new instrument will be equipped with (1) an ESI ion source, an ideal ‘soft’ ionization technique for polar organic molecules and metallocomplexes; (2) a time-of-flight mass spectrometer that can provide high mass resolving power (>20,000) and high mass accuracy (<1 ppm) for compounds ranging from 100 – 10,000 Da; (3) a high performance liquid chromatograph to enable separation of mixtures and allow self-serve by trained HQP; (4) advanced software to allow rapid molecular formula determination. This instrument will be used for experiments across our department, including accurate mass measurement for elemental composition for polar organic compounds and metal complexes derived from synthetic and biochemical studies; structure elucidation of natural products; molecular weight determination of mid-sized molecules; and analysis of reaction mixtures. Because these experiments are critical to research groups across the department, UBC Chemistry has deemed this purchase its highest priority equipment purchase and will contribute substantial funds beyond the $150,000 requested from the NSERC RTI Program. Applicants and co-applicants supporting this application are Raymond Andersen (natural products isolation), Marco Ciufolini (synthetic chemistry), Gregory Dake (synthetic chemistry), Frederic Menard (UBC-Okanagan, synthetic chemistry and neurobiology), Christopher Orvig (medicinal inorganic chemistry), Katherine Ryan (biosynthesis), Paul Schaffer (TRIUMF, radiotracer imaging), and Stephen Withers (glycochemistry and biology). Collectively, we currently train >80 trainees in our individual groups, supported by NSERC Discovery, NSERC Create, NSERC-CIHR CHRP, and other grants. The purchase of this instrument is critical to the success of our research programs.