Ultra-High Performance Liquid Chromatography as a High-Throughput Analytics Platform for Organic Chemistry

高通量实验的出现，即可以同时进行数百到数千个实验，改变了许多物理和生命科学学科进行研究的方式。有机合成是一个采用高通量方法进展缓慢的领域，尽管有很大的发展潜力。虽然近年来工业界越来越多地使用高通量方法进行反应优化和平行合成，但学术研究大多保持迭代和线性方法：每次改变一个因素进行优化，导致在烧瓶中进行的单一实验中合成。持续的进步需要我们的方法和工具的现代化。利用我过去在制药过程化学方面的经验，Leitch集团 * 将开发新型的高通量实验，最终 * 为学术和工业研究人员带来进步。高通量 * 工作流程最重要的部分是快速、可靠且用户友好的分析平台。本 * 提案旨在为一台超高效液相色谱 * 仪器提供资金，该仪器每天可进行1，400次以上的分析。自动化采样可实现 *24/7操作，自动化数据处理软件可整理大 * 数据集。据我所知，在加拿大西部的一所大学里，没有一个系统具有这种水平的吞吐量。这是一个独特的培训机会：学生将成为尖端分析平台的专家，并使用现代数据科学和统计技术处理大型数据集。此外，使用这一 * 仪器将对区域工业实验室极具吸引力，从而促进学术/工业合作。该仪器是新的 *Leitch集团计划的基础，该计划将于2019年1月在UVic进行。该项目旨在根据通过高通量实验获得的数据集开发化学反应性的预测模型。这是一个新的竞争激烈的领域，学术和工业实验室最近发表了备受瞩目的研究。因此，确保这一仪器的安全至关重要;没有它，利奇集团的研究计划将是不可能的。这项提案也得到了UVic四位资深同事的共同申请人支持，他们的研究项目将通过这项技术大大加速。我设想这种仪器作为一个核心能力 * 在一个新兴的中心，高通量化学，可用于利益 * 的所有UVic研究人员。

The advent of high-throughput*experimentation, where hundreds-to-thousands of experiments can be run simultaneously, has changed the way many physical and life sciences disciplines*conduct research. One area that has been slow to adopt high-throughput methods,*despite significant potential for advancement, is organic synthesis. While*industry has increasingly used high-throughput approaches for reaction*optimization and parallel synthesis in recent years, academic research has*mostly kept an iterative and often linear approach: optimization by varying one*factor at a time, leading to synthesis in single experiments performed in*flasks. Continued progress requires modernization of our methods and tools.*Leveraging my past experience in pharmaceutical process chemistry, the Leitch group*will develop new kinds of high-throughput experimentation that will ultimately*lead to progress for both academic and industrial researchers.*** The most important part of high-throughput*workflows is a rapid, reliable, and user-friendly analytical platform. This*proposal seeks funding for an Ultra High Performance Liquid Chromatography*instrument capable of more than 1,400 analyses/day. Automated sampling enables*24/7 operation, and automated data processing software collates large*data sets. To my knowledge, no system with this level of throughput exists at a Western Canadian university.*This represents a unique training opportunity: students will become*experts on a cutting-edge analytical platform, as well as handle large*data sets using modern data science and statistical techniques. In addition, access to this*instrument will be highly attractive to regional industrial labs, thus fostering academic/industry collaborations.*** This instrument is foundational to the new*Leitch group program, which will get underway at UVic in January 2019. This*program seeks to develop predictive models for chemical reactivity based on*data sets obtained via high-throughput*experimentation. This is a new and competitive field, with academic and*industrial labs recently publishing high-profile studies. It is therefore*critical to secure this instrument; without it, the Leitch group research*program would be impossible. This proposal also has co-applicant support from*four senior colleagues at UVic whose research programs will be significantly*accelerated by this technology. I envision this instrument as a core capability*in an emerging center for high-throughput chemistry, available for the benefit*of all UVic researchers.