A portable in vivo metabolic and blood analysis system for field and laboratory work

用于现场和实验室工作的便携式体内代谢和血液分析系统

• 批准号: RTI-2020-00024
• 负责人: Pamenter, Matthew
• 金额: $ 3.18万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=688756
• 关键词: portable; vivo; metabolic; blood; analysis

Reduced oxygen availability (i.e., hypoxia) poses a difficult challenge to most vertebrates and is a central component of many clinically-relevant diseases and pathologies (e.g., heart attack, stroke, chronic pulmonary disorders, etc.). There are numerous hypoxic environments on earth and animals that live in these niches have evolved unique adaptations to meet the challenges of life in hypoxia. Unfortunately, our understanding of the systemic control and evolutionary origins of these adaptations in hypoxia-tolerant mammals is poor. Investigating physiological adaptations to hypoxia and their underlying control in a wide range of phylogenetically distinct mammals is critical to understanding the evolutionary origins of hypoxia-tolerance. To address this knowledge gap, the Pamenter laboratory studies naturally-evolved mechanisms of hypoxia-tolerance in mammals, with an emphasis on the brainstem control centers that regulate ventilation and metabolism in hypoxia. Critically, many of the most hypoxia-tolerant species are not commercially available and must be studied in the field, often in very remote locations. Conducting field work under these conditions requires specialized equipment.******The equipment requested in this RTI application comprises 1) a highly portable, durable, and self-contained (including power supply and data storage) gas detection system for the measurement of whole animal respiratory gases as an indirect but accurate measure of metabolic rate, and 2) an equally portable blood analyzer capable of measuring blood-gas properties, acid-base status, and other metabolites. With this system, HQP from the Pamenter laboratory will be able to test novel hypotheses regarding adaptations to hypoxia by studying rare and difficult to reach species that likely have unique adaptations to life in hypoxia but which have never been previously studied, such as those native to the Tibetan plateau and the plains of South Africa. ******The requested system represents the state of the art in portable metabolic investigations suitable for in vivo use in awake and freely behaving animals, and also offers considerable cost-savings relative to modular benchtop systems. More importantly, this system is durable and portable and is therefore suitable for use in both demanding field settings as well as in a classic university laboratory setting. The system will be maintained and supported by the operating grants of Dr. Pamenter and will be used immediately, and on a daily basis, by HQP from his group and other research groups at the University of Ottawa. It will also be critical to the development and expansion of several ongoing international collaborations. HQP-driven research supported by this equipment is expected to advance our knowledge of the mechanisms and evolutionary origins of mammalian adaptation to hypoxia, and also to inform clinical strategies for the treatment of hypoxia-related illnesses and pathologies.**

减少的氧气利用率（即缺氧）对大多数脊椎动物构成了艰巨的挑战，并且是许多与临床相关的疾病和病理学（例如，心脏病发作，中风，慢性肺部疾病等）的核心组成部分。地球上有许多缺氧环境，生活在这些壁ni中有多种疾病，已经发展出独特的适应性，以应对低氧生活的挑战。不幸的是，我们对缺氧哺乳动物这些适应的系统控制和进化起源的理解很差。研究对低氧及其在多种系统发育不同的哺乳动物中的生理适应性及其基础控制对于理解低氧耐受性的进化起源至关重要。为了解决这一知识差距，调光器实验室研究在哺乳动物中自然发展的耐受耐受性的机制，重点是调节缺氧的通风和代谢的脑干控制中心。至关重要的是，许多最缺氧的物种无法商业上可用，并且必须在野外进行研究，通常是在非常偏远的位置。在这些条件下进行现场工作需要专门的设备。******在此RTI应用中要求的设备包括1）高度便携式，耐用且具有自动化（包括电源和数据存储）的气体检测系统，用于测量整个动物呼吸器，作为间接量的含量和适应性的血液分析量，以衡量全部动物呼吸道的量度，并准确地衡量了能力的血液分析。有了该系统，调子剂实验室的HQP将能够通过研究罕见和难以到达可能对低氧生命的独特适应性的物种来检验有关适应缺氧的新假设，而这些物种以前从未被研究过，例如藏族高原和南非平原的原产性。 ************在便携式代谢研究中，代表适合在醒着和自由表现动物体内使用的可移植代谢研究中的最新技术，还提供相对于模块化台式系统的相对于模块化台式系统提供的相当大的成本节省。更重要的是，该系统耐用且便携，因此适合在苛刻的现场设置以及经典的大学实验室环境中使用。该系统将得到Pamenter博士的运营赠款的维护和支持，并将在他的小组和渥太华大学的小组和其他研究小组的HQP立即使用。这对于几项正在进行的国际合作的发展和扩展也将是至关重要的。该设备支持的HQP驱动的研究有望促进我们对哺乳动物适应缺氧的机制和进化起源的了解，并为治疗与缺氧相关疾病和病理学的治疗临床策略提供信息。** **