COVID-19: Portable Raman Analyzer for point-of-care rapid diagnosis of COVID-19

COVID-19：便携式拉曼分析仪，用于即时诊断 COVID-19

• 批准号: 554386-2020
• 负责人: Venkatakrishnan, Krishnan
• 金额: $ 3.64万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=704859
• 关键词: COVID; 19; Portable; Raman; Analyzer

Rapid diagnosis of viral infection is critical for the prevention of virus spreading. Currently there are approximately 30 FDA-approved COVID-19 test kits on the market. All of them are based on a PCR process and must be operated by laboratories certified for high or moderate-complexity tests. The process is time consuming and usually takes hours to obtain testing results. Test kits for rapid diagnosis emerged in the market in April 2020, but the maximum number of tests that can be performed is only 15 per day. Ryerson researchers in collaboration with Neuronic Works propose a COVID-19 test kit that has the potential to cut the test time to 2 minutes. The proposed test kit consists of a portable Raman Analyzer and quantum-probe-based test medium. Three commonly used COVID-19 markers will be tested. The Ryerson research team has already demonstrated the test kit for the detection of cellular DNA with limitation of detection down to femtomolar concentration. The current project of enabling detection of COVID-19 RNA, proteins and antibodies is a natural extension of their earlier research. The team will conduct a proof-of-concept study and develop algorithms and logics for accurate computerized diagnosis. Neuronic Works, one of the leading companies in electronic device design, will transfer this laboratory research into a prototype COVID-19 test kit. A test kit like this could safeguard public health and safety and reduce the spread of COVID 19 since infected cases (including mild and asymptomatic cases) could be quickly identified. As a result, a broader opening of the economy is possible.

病毒感染的快速诊断对于预防病毒传播至关重要。目前市场上约有30种FDA批准的COVID-19检测试剂盒。所有这些都是基于PCR过程，必须由经过认证的实验室进行高或中等复杂性的测试。这个过程很耗时，通常需要几个小时才能获得测试结果。2020年4月，市场上出现了用于快速诊断的检测试剂盒，但每天最多只能进行15次检测。 瑞尔森的研究人员与Neuronic Works合作，提出了一种COVID-19检测试剂盒，有可能将检测时间缩短到2分钟。 该试剂盒由便携式拉曼分析仪和基于量子探针的测试介质组成。将检测三种常用的COVID-19标志物。瑞尔森研究小组已经展示了用于检测细胞DNA的检测试剂盒，其检测范围限制为飞摩尔浓度。 目前能够检测COVID-19 RNA、蛋白质和抗体的项目是他们早期研究的自然延伸。该团队将进行概念验证研究，并开发算法和逻辑，以实现准确的计算机诊断。 电子设备设计领域的领先公司之一Neuronic Works将把这项实验室研究转化为COVID-19测试套件原型。 像这样的检测试剂盒可以保障公共卫生和安全，并减少COVID 19的传播，因为感染病例（包括轻度和无症状病例）可以快速识别。因此，更广泛的经济开放是可能的。