Continuous Culture of Hybridoma Cells

本研究的具体目的是：系统地研究杂交瘤细胞在连续培养中的生长动力学，并检验Monod模型在不同生长限制基质下对该系统的适用性；阐明生长、抗体产生和各种环境参数变化率之间的功能关系。我们将特别关注细胞能量代谢对这些关系的影响。该研究还旨在检查在批量培养中发生细胞猝死的原因，并探讨其与文献中报道的低稀释率下较低存活率的关系；并应用上述研究获得的动力学信息来操纵细胞在培养中的生长和生产，使其朝着最佳路径发展。本研究将促进对杂交瘤细胞生长的认识。本研究解决的问题与工艺技术有关。本研究获得的动力学信息将对该领域的生物学研究起到补充作用。

The specific aims of the research are as follows: to systematically study the growth kinetics of hybridoma cells in continuous culture and to examine the applicability of the Monod model to the system under different growth limiting substrates; to elucidate the functional relationship among growth, antibody production, and rates of change of various environmental parameters. Special attention will be given to the effect of cellular energy metabolism on these relationships. The research also aims to examine the cause of sudden cell death occurring in batch culture and to explore its relationship to the lower viability at low dilution rates reported in the literature; and to apply the kinetic information obtained from the above studies to manipulate cell growth and production in the culture toward an optimum path. This research will advance the understanding of the growth of hybridoma cells. The problems addressed by this study are related to process technology. The kinetic information obtained from this study will complement the biological studies in this field.