Enhanced Enzyme Productivity in Microorganisms Experiencing Prolonged System Transients

说明：酶是细胞内的蛋白质，可作为特定生化反应的生物催化剂。生产大量用于分析、诊断或治疗的酶是生化工业的主要关注点。酶是通过在最佳生长条件下培养微生物，收集微生物，并提取和纯化所需的蛋白质来产生的。目前，两类酶，酒精氧化酶和葡萄糖氧化酶，商业上使用的是酵母，多形汉逊酵母，在连续培养中使用单一的碳能底物；甲醇或葡萄糖。过去的工作结果表明，如果以较低的生长速度使用葡萄糖和甲醇的混合物，这两种酶的水平都可以增加一倍或三倍。这项研究旨在扩展上述稳态工作，以研究系统生长速度和底物碳混合物中规定的变化对酶生产率的影响。结果将增加我们对瞬变条件下微生物调控过程的理解。

Narrative: Enzymes are proteins contained within cells that serve as biological catalysts of specific biochemical reactions. Production of a myriad of enzymes for both analytical, diagnostic, or therapeutic uses is a major concern of the biochemical industry. Enzymes are produced by cultivating microorganisms under optimum growth conditions, collecting the microbe, and extracting and purifying the desired protein. Currently, two classes of enzymes, alcohol- and glucose-oxidizing enzymes, are produced commercially using the yeast, Hansenula polymorpha, grown in continuous culture using a single carbon energy substrate; either methanol or glucose. Results of past work indicate levels of both enzymes can be doubled or tripled if a mixture of glucose and methanol is used at low growth rate. This research intends to extend the above steady-state work to investigate effects of prescribed shifts in both system growth rate and substrate carbon mixtures on enzymes productivity. Results will increase our understanding of microbial regulatory processes under transient conditions.