Use of Insect Cell Culture for Production of Proteins and Viral Pesticides

昆虫细胞-杆状病毒系统 迅速而广泛地受到 作为实验室或小型实验室- 规模化生产系统 来自重组DNA的蛋白质，和 一种由昆虫生产的“艾滋病疫苗” 细胞系统正在进行临床试验 该系统具有以下优点： 安全性高，生产率高， 可靠生产活性 蛋白质，以及高水平的 翻译后加工 由于翻译后 昆虫细胞加工机械 与我们所知的 哺乳动物细胞中， 蛋白质种类的限制 最好是在昆虫中生产的 细胞系统 然而，产品 从昆虫细胞中通常会引出 强烈的免疫原性应答，和 该系统特别显示 非常适合用于疫苗 生产和可能 抗体的 此外，病毒本身可能是 经过基因改造以改善 它作为一种安全的，特异的， 有效的杀虫剂， 主要农作物病虫害 害虫 的一个主要 研究者（PI）进行了 在这个国家的第一次测试 从基因上释放的能量场 工程病毒 本报告的总体目标 项目是一个发展 可预测的大规模 生产蛋白质和 昆虫细胞杀虫剂 线 无论是生产 蛋白质或病毒杀虫剂，更多 高效的生产系统， 必需的. 因为这些产品 可能需要大量的， 而且很可能对成本敏感 (e.g.,动物疫苗和 生物杀虫剂）， 系统将强烈依赖于 改进和新颖的工程 设计

The insect cell-baculovirus system has received rapid and wide acceptance as a laboratory or small- scale system for production of proteins from recombinant DNA, and an "AIDS vaccine" produced by insect cell systems is in clinical trials. This system offers advantages of safety, high productivity, generally reliable production of active proteins, and a high degree of post-translational processing. Since the post-translational processing machinery of insect cells is not identical to that of mammalian cells, there may be some limitation on the types of proteins that are best produced in insect cell systems. However, products from insect cells typically elicit a strong immunogenic response, and this system appears particularly well-suited for use in vaccine production and possibly for antibodies. In addition, the virus itself may be genetically-engineered to improve its properties as a safe, specific, and effective insecticide against major crop pests and forest insect pests. One of the Principal Investigators (PIs) has conducted the first tests in this country of a field released genetically- engineered virus. The overall objective of this project is the development of a basis for predictable large-scale production of proteins and insecticides using insect cell lines. For either production of proteins or viral pesticides, more efficient production systems will be required. Because these products may be required in large volumes, and are likely to be cost-sensitive (e.g., animal vaccines and bioinsecticides), the success of the system will be strongly dependent on improved and novel engineering design.