600 MHz NMR Spectrometer for Structure Determination of Proteins and RNA

科罗拉多大学博尔德分校化学和生物化学系的生物化学部将购买一台最先进的四通道600 MHz核磁共振波谱仪，NSF多用户生物设备计划将提供部分支持。该仪器用于进行多核多维核磁共振实验，用于同位素标记的蛋白质和RNA的溶液结构测定。三重共振多维核磁共振技术现在允许蛋白质的完全通过键共振指定，这意味着蛋白质溶液结构确定(共振指定)的主要瓶颈现在可以比以前的技术更明确和更少的时间。现代蛋白质的NM-R溶液结构测定需要同位素标记的蛋白质和具有脉冲整形和脉冲场梯度能力的三重共振仪。多核多维核磁共振技术在蛋白质的溶液结构测定中已被证明是如此成功，现在可以应用于RNA的结构研究和蛋白质-RNA相互作用的研究。这个额外的核磁共振仪器是必需的，以满足生物化学系最近扩充的教员(伍特克博士)对核磁共振时间的需求。所要求的仪器将极大地提高核磁共振能力，并将加速以下领域的研究：-RNA和蛋白质-RNA系统的溶液结构研究(PARDI)-确定RNA溶液结构的核磁共振技术的发展(PARDI)-心肌肌钙蛋白C(FALKE)的核磁共振结构研究-I族核酶活性部位的结构研究(CHECH)-催化RNAs的结构和功能(Uhlenbeck)-反式作用病毒蛋白Tax、Px及其复合体的溶液结构研究(Wuttke)

The Biochemistry Division in the Department of Chemistry and Biochemistry at the University of Colorado, Boulder will purchase a state-of-the-art four channel 600 MHz NMR spectrometer with partial support from the NSF Multi-User Biological Equipment program. This instrument is required to perform multi-nuclear multi-dimensional NMR experiments used in the solution structure determinations of isotopically labeled proteins and RNAs. Triple-resonance multi-dimensional NMR techniques now allow complete through bond resonance assignment of proteins which means that the primary bottleneck in solution structure determinations of the proteins (the assignment of the resonances) can now be made much more unambiguously and in much less time than previous techniques. The modern NM R solution structure determinations of a protein requires isotopically labeled protein and access to a triple-resonance instrument that has pulse shaping and pulse-field gradient capabilities. The multi-nuclear and multi-dimensional NMR techniques that have proved so successful in solution structure determinations of proteins can now be applied to structural studies of RNAs and protein-RNA interactions. This additional NMR instrumentation is required to meet the demand for NMR time arising from the recent expansion of the faculty (Dr. Wuttke) in the Biochemistry Divisimn. The requested instrumentation will vastly improve the NMR capabilities and will accelerate research in the following areas: - Solution Structural Studies of RNA and Protein-RNA Systems (Pardi) - Development of NMR Techniques for Solution Structure Determinations of RNA (Pardi) - NMR Structural Studies of Cardiac Troponin C (Falke) - Structural Studies of the Active Site of the Group I Ribozyme (Cech) - Structure and Function of Catalytic RNAs (Uhlenbeck) - Solution Structural Studies of the trans Acting Viral Proteins Tax, pX and Their Complexes (Wuttke)