Biocatalysis Near and Above 100 degrees Celsius: Physiological, Enzymological and Engineering Studies

这一提议是关于研究两种模式的超嗜热细菌——古细菌furiosus Pyrococcus （Tmax 105℃）和细菌Thermotoga martima （Tmax 90℃）——的酶的基础和应用问题。这将使用两种生物的全基因组序列信息来完成，这些信息将在项目过程中提供。此外，将审查深海和大陆地热地点的超嗜热菌的多样性，并研究它们所含的酶的类型，以研究它们的生理意义、热稳定性/热活性和/或生物技术潜力。重点将放在水解酶和氧化还原酶参与碳水化合物和肽的代谢。该酶组的多样性和生化特征将通过重组和生化技术来研究，以改变底物特异性的模式。超嗜热酶的热稳定和化学稳定机理也将被讨论。此外，将结合工业合作，评估现有和新酶在各种生物技术应用中的使用情况。＊＊＊

9632657 Kelly This proposal is on research to examine both basic and applied issues of enzymes from two model hyperthermophiles, the archaeon Pyrococcus furiosus (Tmax 105 C) and the bacterium Thermotoga maritima (Tmax 90 C). This will be done using sequence information on the entire genomes of both organisms which will be available during the course of the project. Also, the diversity of hyperthermophiles in both deep sea and continental geothermal sites will be examined, and the types of enzymes they contain will be studied for their physiological significance, thermmostability/thermoactivity and/or biotechnological potential. Emphasis will be placed on hydrolytic and redox enzymes involved in the metabolism of carbohydrates and peptides. Characterization of the diversity and biochemical features of this enzyme group will be investigated by both recombinant and biochemical techniques to change patterns of substrate specificity. Mechanisms of thermostabilization and chemical stabilization of hyperthermophilic enzymes will also be addressed. Also, the use of both existing and new enzymes will be evaluated in a variety of biotechnological applications in conjunction with an industrial collaboration. ***