Ethylene and Pathogen Infection Responses in Tomato and Arabidopsis

9728133 Klee植物和致病病原体的相互作用通常分为两种类型：相容和不相容。 不相容导致抗性，而相容导致疾病。 在没有抗性反应的情况下，疾病症状可以传播，导致植物的严重损害或死亡。 使用乙烯不敏感的番茄突变体，我们已经证明了这种植物激素在兼容番茄的疾病症状发展中的核心作用：病原体相互作用。 我们打算，通过使用突变体，以确定如何乙烯控制疾病的发展，在兼容的植物：病原体的相互作用。 实验将在分子遗传水平上确定乙烯在番茄症状发展中的作用：黄单胞菌相互作用。 利用乙烯合成或感知改变的植物以及水杨酸（SA）积累不足的植物，PI将表征乙烯和SA在调节疾病早期和晚期阶段中的作用。 PI还将研究拟南芥中的相容性相互作用。 了解乙烯不敏感性如何减弱多种病原生物的症状发展，也提供了广泛和持久的宿主耐受性的可能性，在农业上具有重要的应用。

9728133 Klee The interactions of plants and disease-causing pathogens generally fall into two types: compatible and incompatible. Incompatibility leads to resistance while compatibility leads to disease. In the absence of a resistant response, disease symptoms can spread leading to severe damage or death of the plant. Using an ethylene-insensitive tomato mutant, we have demonstrated a central role for this phytohormone in disease symptom development in compatible tomato:pathogen interactions. We intend, through the use of mutants, to define how ethylene controls disease development in compatible plant:pathogen interactions. Experiments will define at a molecular genetic level the role of ethylene in symptom development in the tomato:Xanthomonas interaction. Using plants altered in ethylene synthesis or perception as well as plants deficient in accumulation of salicylic acid (SA), the PIs will characterize the roles of ethylene and SA in regulating early and advanced stages of disease. The PIs will also examine a compatible interaction in Arabidopsis. An understanding of how ethylene insensitivity attenuates symptom development to multiple pathogenic organisms also offers the possibility of broad and durable host tolerance with significant applications to agriculture.