Ethylene and Pathogen Infection Responses in Tomato and Arabidopsis

9728133 Klee植物与致病病原体的相互作用一般分为两种类型：亲和性和不亲和性。不相容导致抵抗力，相容导致疾病。在缺乏抗性反应的情况下，疾病症状可能会传播，导致植物受到严重损害或死亡。利用一个乙烯不敏感的番茄突变体，我们已经证明了这种植物激素在亲和番茄的疾病症状发展中的核心作用：病原菌相互作用。我们打算通过使用突变体来定义乙烯如何控制亲和植物中的疾病发展：病原体相互作用。实验将在分子遗传水平上定义乙烯在番茄症状发展中的作用：黄单胞菌的相互作用。利用乙烯合成或感知改变的植物以及缺乏水杨酸(SA)积累的植物，PI将表征乙烯和SA在调节疾病早期和晚期的作用。PI还将检查拟南芥中的相容交互作用。对乙烯不敏感如何减轻对多种病原体的症状发展的理解，也提供了广泛和持久的寄主耐受性的可能性，并在农业上有重要的应用。

9728133 Klee The interactions of plants and disease-causing pathogens generally fall into two types: compatible and incompatible. Incompatibility leads to resistance while compatibility leads to disease. In the absence of a resistant response, disease symptoms can spread leading to severe damage or death of the plant. Using an ethylene-insensitive tomato mutant, we have demonstrated a central role for this phytohormone in disease symptom development in compatible tomato:pathogen interactions. We intend, through the use of mutants, to define how ethylene controls disease development in compatible plant:pathogen interactions. Experiments will define at a molecular genetic level the role of ethylene in symptom development in the tomato:Xanthomonas interaction. Using plants altered in ethylene synthesis or perception as well as plants deficient in accumulation of salicylic acid (SA), the PIs will characterize the roles of ethylene and SA in regulating early and advanced stages of disease. The PIs will also examine a compatible interaction in Arabidopsis. An understanding of how ethylene insensitivity attenuates symptom development to multiple pathogenic organisms also offers the possibility of broad and durable host tolerance with significant applications to agriculture.