Instrumentation for a Center for Genetic Diversity and Gene Expression Profiling at the Boyce Thompson Institute and Cornell University

摘要本项目涉及到在康奈尔大学博伊斯汤普森研究所建立遗传多样性和基因表达谱研究中心的仪器设备的购置和安装。 要获得的仪器包括用于cDNA微阵列和扫描微阵列的机器人系统，用于自动化生产用于高通量荧光测序和基因分型的DNA模板的ABI 377热循环仪，以及用于快速分离和定量DNA片段以鉴定SNP的Transgenomic WAVE DNA片段分析系统。 该仪器将被放置在一个共享的用户设施，以支持参与旨在了解植物遗传多样性和全基因组基因表达的研究的大量PI。 这些设施将支持9个主要用户和一些次要用户的研究项目。 所要求的仪器将支持对理解遗传多样性和全基因组基因表达的共同关注。 与基因表达谱有关的仪器将设在博伊斯汤普森研究所，而与DNA序列多样性检测有关的设备将设在康奈尔生物技术大楼。 研究将通过中心主任进行协调，以便为用户的研究和培训活动以及仪器的维护提供最佳支持。

Abstract This project involves the acquisition and installation of instrumentation to establish the Center for Genetic Diversity and Gene Expression Profiling at the Boyce Thompson Institute at Cornell University. Instrumentation to be acquired includes a robotics system for cDNA microarraying, and scanning microarrays, an ABI 377 thermal cycler to automate production of DNA templates for high throughput fluorescent sequencing and genotyping, and a Transgenomic WAVE DNA fragment analysis system for rapid separation and quantification of DNA fragments to identify SNPs. The instrumentation will be placed in a shared user facilities to provide support for a large number of PIs involved in research aimed at understanding genetic diversity and genome-wide gene expression in plants. These facilities will support the research projects of 9 major users and a number of minor users. The instrumentation requested will support a common focus on understanding genetic diversity and genome-wide gene expression. Instrumentation related to gene expression profiling will be housed at the Boyce Thompson Institute whereas equipment related to the detection of DNA sequence diversity will be housed in the Cornell Biotechnology building. The research will be coordinated through the Directors of the Center to provide optimum support of the research and training activities of the users and maintenance of the instrumentation.