Acquisition of a MALDI-TOF Mass Spectrometer for Analysis of the Proteome of the Model Plant, Arabidopsis Thaliana

资金要求购买MALDI-TOF（基质辅助激光解吸-飞行时间）质谱仪，这将是一个多用户的研究设施在威斯康星大学麦迪逊分校在植物蛋白质组学领域。 具体来说，这台仪器将被十几个建立良好的联邦资助的实验室在校园里进行基因组研究的模式高等植物，拟南芥。 质谱仪将作为UW生物技术中心现有肽质谱设施的一部分运行。 该仪器将对植物基因组学的校园基础研究产生巨大影响，使我们能够快速识别蛋白质和肽的精确分子量，以及它们的修饰形式，并促进正在进行的植物基因功能鉴定研究。 拟南芥基因组测序计划是ca。目前已经完成了50%，预计在未来两年内完成整个100兆基因组。 完整序列的知识使我们能够以非常节省时间和资源的方式利用质谱仪，并为探索植物生长和发育的分子基础开辟了新的途径。

Funds are requested to purchase a MALDI-TOF (matrix assisted laser desorption-time of flight) mass spectrometer, that will be operated as a multi-user facility for research at UW-Madison in the area of plant proteomics. Specifically, this instrument will be used by a dozen well established federally funded laboratories on campus performing genomic research in the model higher plant, Arabidopsis thaliana. The mass spectrometer will be operated as part of the UW Biotechnology Center's existing peptide mass spectrometry facility. This instrument will have a dramatic impact on campus basic research in plant genomics, in allowing us to rapidly identify the precise molecular weight of proteins and peptides, as well as their modified forms, and facilitate ongoing studies in the identification of plant gene function. The Arabidopsis genome sequencing project is ca. 50% completed already, and the entire 100 Megabase genome is expected to be completed within the follow two years. Knowledge of the complete sequence allows us to utilize the mass spectrometer in a very time and resource efficient manner and opens new avenues for exploring the molecular basis for plant growth and development.