Acquisition of an Electrospray Ionization Mass Spectrometer for the Shared Instruments Facility of the Johns Hopkins University

9977581meyer主要研究仪器项目的支持将用于获得一台配备高压液相色谱仪的离子阱电喷雾电离质谱仪。这两种仪器都将被安置在仪器设施中，这是化学系主要仪器的共享核心资源。所要求的光谱仪将是约翰霍普金斯大学霍姆伍德校区唯一的同类仪器。该光谱仪将允许高分子量测定，复杂混合物的原位分析，液相色谱-质谱分析，结构测定和弱结合分子或生物组装的表征。来自化学或生物物理化学的研究人员将是该仪器的主要用户，其他九名霍普金斯大学的教职员工将是次要用户。研究项目包括超分子无机配位化合物、铜与血红素/铜的配位复合物、葡萄球菌核酸酶蛋白的折叠等。该光谱仪将完全供大学所有部门的研究小组使用。值得注意的是，这些仪器将与巴尔的摩地区姐妹机构的研究人员共享，这些研究人员非常依赖霍普金斯大学的仪器设施提供支持。主要研究仪器基金将用于购买离子阱电喷雾电离质谱仪，该质谱仪将用于涉及大分子的各种研究项目。该仪器将由大学所有部门的一大组研究人员以及姐妹机构的研究人员使用。仪器将影响学生的研究训练，因为它将给他们宝贵的实践经验，在质谱技术。

9977581MeyerSupport from the Major Research Instrumentation program will be used to acquire an ion trap electrospray ionization mass spectrometer equipped with a high-pressure liquid chromatograph. Both instruments will be housed in the Instruments Facility, a shared core resource for major instrumentation located in the Department of Chemistry. The requested spectrometer will be the only instrument of its kind at the Homewood Campus of the Johns Hopkins University. The spectrometer will allow high molecular weight determinations, in situ analysis of complex mixtures, liquid chromatography-mass spectrometry, structural determination, and characterization of weakly bound molecular or biological assemblies. Investigators from chemistry or biophysical chemistry will be major users of this instrumentation, and nine other Hopkins faculty members will be minor users. The research projects range from supramolecular inorganic coordination compounds, coordination complexes of copper and heme/copper, to folding of staphococcal nuclease protein. The spectrometer will be fully accessible to research groups in all divisions of the University. Significantly, the instrumentation will be shared with researchers at sister institutions in the Baltimore area who greatly rely on the Instruments Facility at Hopkins for support. Major Research Instrumentation funds will be used to acquire an ion trap electrospray ionization mass spectrometer that will be used in a variety of research projects that involve large molecules. The instrumentation will be used by a large group of researchers, within all the divisions of university, as well as by researchers at sister institutions. The instrumentation will impact students in research training as it will give them valuable hands-on experience in mass spectroscopic techniques.