Quantitative und funktionelle Analysen der Proteomveränderungen während chronischer Virusinfektionen (Z03 (A05))

中央项目被整合到更大的项目中，作为蛋白质组学和原代细胞培养的服务平台，可供所有合作伙伴使用。在蛋白质组学部分，将提供生物分析服务，包括从样品制备到最先进的质谱分析的方方面面。此外，与蛋白质组学有关的生物信息学和统计服务的实施计划在拟议的供资期间进行。该项目的第二项任务将是提供来自内脏器官的原代细胞以及来自人类和小鼠的免疫细胞。所提供的固有层聚集体培养模型适用于人类免疫缺陷病毒的体外高效感染。此外，还提供了一种体外感染模型，在该模型中，使用细胞培养衍生的乙肝病毒感染原代人肝细胞。除了这些服务，这个项目还嵌入了一个广泛的科学合作网络。

The central project is integrated in the bigger project as a service platform for proteomics and primary cell cultures, available for all collaboration partners. In the proteomics part the bioanalytics services, implying all aspects starting from sample preparation to state-of-the-art mass spectrometric analyses will be offered. Additionally, the implementation of proteomics-related bioinformatics and statistics services is intended in the proposed funding period. The second task of the project will be to provide primary cells from visceral organs as well as immune cells from humans and mice. The lamina propria aggregate culture model that will be provided is suitable for efficient human immunodeficiency virus infection in vitro. Furthermore, an in vitro infection model is offered, in which cell-culture-derived Hepatitis B viruses are used to infect primary human hepatocytes. In addition to the services, this project is also imbedded in a broad network of scientific collaborations.