Photodissociation in a Quadrupole Ion Trap

德克萨斯大学奥斯汀分校的詹妮弗·布罗德贝特教授将与北得克萨斯大学的艾伦·马钱德教授合作，开展一个由分析和表面化学计划支持的生物分析化学项目。本项目旨在研究和改进用于多肽和蛋白质分析的四极离子陷阱质谱仪中的光解离方法。方法是将分析物物种与金属离子和/或有机配体络合，以更有效地吸收来自光解离激光的光子，从而将足够的能量转移到分析物以用于碎裂。还将探索优化光解离用于高效液相色谱(HPLC)分析。拟议的工作结合了布罗德贝特教授在质谱学(MS)和离子化学方面的专业知识，以及马钱德教授在合成和大环化学方面的专业知识。布罗德贝特教授的团队已经就拟议研究的两个组成部分发表了广泛的文章-第一，有机和生物化合物的光解离，第二，通过形成络合物来增强离子活化(碰撞诱导解离)。目前的工作将这两条线的研究结合在一起，并将它们扩展到涉及高效液相色谱-质谱仪的潜在应用。多肽和蛋白质的高效液-质联用分析是蛋白质组学的重要工具，但将受益于这里探索的改进的离子激活技术。与Marchand教授的合作允许交叉培训，这对分析化学和有机化学小组的学生都是有价值的。Marchand教授的团队将合成用于络合的新配体。

Professor Jennifer Brodbelt of the University of Texas Austin will work with Professor Alan Marchand of the University of North Texas on a project in bioanalytical chemistry supported by the Analytical and Surface Chemistry Program. This project aims to study and improve photodissociation methods in the quadrupole ion trap mass spectrometer for analysis of peptides and proteins. The approach will be to complex the analyte species with metal ions and/or organic ligands that will more effectively absorb photons from the photodissociation laser and thus transfer sufficient energy to the analyte for fragmentation. Optimization of photodissociation will also be explored for high-performance liquid chromatography (HPLC) analysis. The proposed work combines the expertise of Professor Broadbelt in mass spectrometry (MS) and ion chemistry, and Professor Marchand in synthesis and macrocyclic chemistry. Prof. Brodbelt's group has published extensively on the two building blocks of the proposed research-first, photodissociation of organic and biological compounds, and second, enhancement of ion activation (collision-induced dissociation) through complex formation. The present work brings these two lines of research together, and extends them to potential applications involving HPLC-MS. HPLC-MS analysis of peptides and proteins is an important tool for proteomics, but would benefit from the improved ion activation techniques explored here. The collaboration with Prof. Marchand, whose group will synthesize novel ligands for complexation, allows cross training that will be valuable for students in both the analytical chemistry and organic chemistry groups.