A Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer Integrated with UPLC for Characterization of Protein Complexes and Comparative Proteomics

该合同为购买混合四极杆飞行时间串联质谱仪（Q-TOF）提供资金，该质谱仪与完全集成的超高压液相色谱（UPLC）系统相连接。该仪器将成为美国国家科学基金会（NSF）六个主要用户（包括康奈尔大学蛋白质组学核心设施）和多个次要用户的研究资助的核心。该仪器将补充蛋白质组学核心设施和植物生物系的现有设备，特别是通过它在分析蛋白质-蛋白质和蛋白质-配体相互作用以及对基因组序列未知的生物体的蛋白质进行从头测序方面的应用。高灵敏度和精确的串联质谱是鉴定和表征低丰度蛋白质的关键。此外，该仪器将成为使用稳定同位素标记进行比较定量（植物）蛋白质组学研究的关键工具。质谱仪将加速和促进康奈尔大学不同院系的广泛研究项目。该仪器还有望帮助培训40多名本科生、研究生和博士后，并将在美国国家科学基金会资助的“植物基因组学”推广计划中发挥作用，该计划吸引高中学生和教师，并影响该地区不同学院的本科生。

This award provides funds to aid in the purchase of a hybrid Quadrupole Time-of-Flight Tandem Mass spectrometer (Q-TOF), interfaced with a fully integrated ultrahigh pressure liquid chromatography (UPLC) system. The instrument will be central to research supported NSF research grants awarded to the six major users, including the Cornell Proteomics Core facility, and multiple minor users. The instrument will complement existing equipment in the proteomics core facility and the Department of Plant Biology, particularly through its application in the analysis of protein-protein and protein-ligand interactions and in de-novo sequencing of proteins from organisms whose genomic sequence is unknown. Highly sensitive and accurate tandem mass spectrometers are critical in the identification and characterization of low abundance proteins. In addition, the instrument will be a key tool for comparative quantitative (plant) proteomics studies using stable isotope labeling. The mass spectrometer will accelerate and facilitate a wide range of research projects of Cornell Faculty across different departments. The instrument is also expected to aid in the training of more than 40 undergraduate, graduate, and postdoctoral students, and will play a role in an NSF-funded outreach program in 'Plant Genomics', a program that attracts high school students and teachers, and impacts undergraduate students from diverse colleges in the region.