Phosphoproteomic analysis of the rice XA21-Mediated Resistance Response

作为固着生物，植物必须不断地保护自己免受潜在的微生物病原体的侵袭。为了保护植物，植物已经发展出有效的防御机制，启动了对潜在病原菌的胞外检测。然而，将病原菌的胞外识别转化为植物对S的抗性的信号转导途径还知之甚少。因此，鉴定和鉴定水稻等谷类作物中的单个信号成分将有助于更广泛地了解所有谷类作物的防御反应。该项目将使用先进的生化方法来识别水稻植株中激活的信号成分，以抵抗导致白叶枯病的细菌病原体。白叶枯病是世界各地水稻的一种毁灭性疾病。将使用反向遗传方法来测试这些新发现的信号成分是否在介导对细菌病原体的抗性方面发挥重要作用。该项目预计将确定至少10-20个新的信号成分，并通过反向遗传学测试10个最有希望的候选分子在抵抗病原体方面的作用。提高对植物如何应对潜在病原体入侵的知识对于提高植物产量和质量至关重要。此外，水稻是农业上重要的谷物的主要基因组学系统。因此，这项研究的结果将直接帮助研究其他谷类作物病原菌胁迫的研究人员。此外，该项目还将用于培训本科生掌握最先进的生化技术。

As sessile organisms, plants must constantly defend themselves from potential microbial pathogens. For protection, plants have developed effective defense mechanisms initiated by the extracellular detection of the potential pathogen. However, the signaling pathways that translate extracellular recognition of pathogens into the plant?s resistance are poorly understood. Therefore, identification and characterization of individual signaling components in a cereal crop such as rice will lead to a broader understanding of the defense response in all cereals. The project will use advanced biochemical approaches to identify signaling components activated in rice plants resistant to the bacterial pathogen that causes bacterial leaf blight, a devastating disease of rice throughout the world. Reverse genetic approaches will be used to test if these newly identified signaling components play a significant role in mediating resistance against the bacterial pathogen. The project is expected to identify at least 10-20 new signaling components and to test by reverse genetics 10 of the most promising candidates for their role in resistance against pathogens. Improving knowledge about how the plant responds to invasion by potential pathogens is critical for improving plant yields and quality. Moreover, rice is the leading genomics system for the agriculturally important cereals. Therefore, results from this study will directly benefit researchers studying pathogen stress in other cereal crops. In addition, the project will be used to train undergraduate students in state of the art biochemical techniques.