CAREER: A new Telemedicine Platform using Incoherent Lensfree Cell Holography and Microscopy On a Chip

0954482 Ozcan数字技术的最新革命性进展以及新颖的图像重建算法和理论可以从根本上改变我们进行细胞显微镜检查、细胞计数和医学诊断的方式。有了这个愿景，该提案做出了系统的努力，突出了这个及时的机会，通过使其不含任何透镜，激光器或其他笨重的光学组件，具有更简单，紧凑和具有成本效益的成像架构，特别适合远程医疗的需求和要求，彻底改变细胞显微镜。通过本方案的非相干无透镜细胞全息片上显微镜平台（即，LUCAS），PI提出通过记录各种细胞类型的单独相位振幅全息图来在数字域中补偿光学组件的复杂性的缺乏。这些细胞全息图还可以在非常大的视场（FOV）上精确重建显微图像，即使在细胞密度高达约40万个细胞/μ L的情况下也具有亚细胞分辨率。将具有成本效益的紧凑型LUCAS成像仪与最先进的手机技术相结合，将为远程医疗创造许多机会，以改善医疗保健，特别是在医疗设施和基础设施极为有限的发展中国家。

0954482OzcanThe recent revolutionary progress in digital technologies together with novel image reconstruction algorithms and theories can fundamentally transform the way that we conduct cell microscopy, cytometry and medical diagnostics. With this vision, this proposal makes a systematic effort to highlight this timely opportunity to revolutionize cell microscopy by making it free of any lenses, lasers or other bulky optical components with a much simpler, compact and cost-effective imaging architecture especially suitable for telemedicine needs and requirements. Through incoherent lensfree cell holography & on-chip microscopy platform of this proposal (i.e., LUCAS), the PI proposes to compensate in the digital domain for the lack of complexity of optical components by recording individual phase & amplitude holograms of various cell types. These cell holograms also yield accurate reconstruction of microscopic images featuring sub-cellular resolution over a very large field of view (FOV) even at cell densities reaching up to ~0.4 Million cells/ìL. Merging cost-effective and compact LUCAS imagers with the state-of-the-art cell-phone technology will create numerous opportunities for telemedicine to improve health care especially in the developing world where medical facilities and infrastructure are extremely limited.