An Integrated Lab-on-a-Transistor for Biological Detection

发达国家和发展中国家（全球卫生竞技场）的传染病管理危机需要快速、易于使用、集成和廉价的诊断装置来检测传染病的病原体，即细菌、病毒。在这个应用程序中，开发一个？晶体管实验室提出了用于细胞捕获和热裂解，以及用于在硅晶体管上进行核酸扩增的超快速技术，其具有扩增产物的直接、快速电检测。将解决使核酸扩增真正成为即时检测的巨大挑战，其中可以在硅传感器阵列上在不到5分钟的时间内获得高准确性和可靠性的结果。为了应对这些挑战，李斯特菌将在三年内被用作模型系统，但该技术平台也可以应用于其他病原微生物。 智力优点：提出的概念，发展一个？晶体管实验室具有以下知识性优点：（a）在微流体通道内使用介电泳将单个细菌集中在线性阵列中的单个场效应晶体管上。然后将探讨使用交流电压在场效应传感器表面上的超局部加热。使用该方法，通过达到95 ℃或更高的温度来进行附着在晶体管表面上的细菌的热裂解，（B）将相同的传感器用作加热器，以使用聚合酶链式反应或滚动电路扩增方法进行核酸扩增反应，以及（c）探索晶体管本身用于PCR产物的无标记电检测的用途。广泛影响：拟议的工作将在硅基生物传感器领域产生广泛的影响，拟议的技术可以为开发护理点传感器提供重大进展。研究生将参与夏季讲座和动手研讨会将在伊利诺伊大学的微型和纳米技术实验室举行。REU夏季支持将被要求雇用更多的本科生在夏季与研究生一起工作的研究项目，这也将有助于发展研究生研究人员的未来管道。

The crisis in the management of infectious disease for the developed world and in the developing world (global health arena) requires rapid, easy to use, integrated, and inexpensive diagnostic devices for the detection of agents of infectious diseases, i.e. bacteria, viruses. In this application, the development of a ?Lab-on-a-Transistor? is proposed for cell capture and thermal lysing, and ultra rapid techniques for performing nucleic acid amplification on silicon transistors with a direct, rapid electrical detection of the amplified products. The grand challenge of making nucleic acid amplification truly a point-of-care test will be addressed, where the results can be obtained with high accuracy and reliability in less than 5 minutes on a silicon sensor array. To address these challenges, the bacteria Listeria monocytogenes will be used as the model system within the three years of this proposed grant but the technology platform can be applied to other pathogenic microorganisms as well. Intellectual Merit: The proposed concept of developing a ?Lab-on-a-Transistor? has the following intellectual merit; (a) concentrating single bacteria on individual field effect transistors with in a linear array using dielectrophoresis within a microfluidic channel. Then ultra-localized heating on the surface of the field effect sensor using an ac voltage will be explored. Using this method, thermal lysing of bacteria that are attached on the surface of the transistors will be performed by achieving temperatures of 95oC or higher, (b) the same sensor would be used as a heater to perform nucleic acid amplification reactions using either a polymerase chain reaction or a rolling circuit amplification method, and (c) exploring the use of the transistors themselves for label free electrical detection of the PCR products. Broad Impact: The proposed work will have a broad impact in the area of silicon based biosensors and the proposed technology could provide significant advances in developing point of care sensors. Graduate students will be involved in summer lecture and hands-on workshops to be held at the Micro and Nanotechnology Laboratory at the University of Illinois. REU summer support will be requested for hiring additional undergraduates to work with the graduate students on the research project during the summer, which will also help to develop a pipeline of graduate student researchers for the future.