MRI: Acquisition of a Liquid Chromatography/Mass Spectrometer for Accurate Mass Determination and Complex Mixture Analysis

从主要研究仪器计划（MRI）的这一奖项和化学研究仪器计划（CRIF）的支持下，教授帕特里克Willoughby从里彭学院和同事Colleen拜伦和罗伯特华莱士将获得液相色谱仪，飞行时间质谱仪能够提供准确的质量（LCMS-TOF-准确的质量）。一般来说，质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。带有液相色谱仪的仪器通过在化合物到达质谱仪之前分离化合物的混合物来提供额外的结构鉴定能力。作为一个主要的本科院校，里彭的教师拥抱本科研究经验作为他们的研究活动的最重要的成果之一。拟议的工具将直接影响大量的学生在三年的项目期间的研究经验，并在未来几年更多。本科研究人员将参加与高中的活动。该奖项旨在加强各级研究和教育，特别是在以下领域：（a）开发涉及酰基自由基和光氧化还原催化的新的有机合成方法;（b）优化邻苯炔与羰基叶立德的1，3-偶极环加成反应的条件;（c）优化邻苯炔与羰基叶立德的1，3-偶极环加成反应的条件;（d）优化邻苯炔与羰基叶立德的1，3-偶极环加成反应的条件。（c）研究活性氧副产品对电子转移蛋白质的影响;及（d）研究脂质分布对轮虫存活的影响及轮虫群落的不适口性。

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professor Patrick Willoughby from Ripon College and colleagues Colleen Byron and Robert Wallace will acquire a liquid chromatograph, time-of-flight mass spectrometer capable of providing accurate mass (LCMS-TOF-accurate mass). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. As a primarily undergraduate institution, Ripon's faculty embraces undergraduate research experiences as one of the most important outcomes of their research activities. The proposed instrument will directly impact the research experiences of a large number of students within the three-year project period, and many more in years to come. Undergraduate researchers will participate in activities with high schools. This instrument will also be shared with researchers from the University of Wisconsin Oshkosh as well as those from Lawrence University.The award is aimed at enhancing research and education at all levels, especially in areas such as (a) developing new organic synthesis methodology involving acyl radicals and photoredox catalysis; (b) optimizing conditions for the 1,3-dipolar cycloaddition reaction of o-benzyne with a carbonyl ylide; (c) study the effects of byproducts of reactive oxygen species on electron-transferring proteins; and (d) studying the effect of lipid profiles on rotifer survival and the unpalatability of rotifer colonies.