Time resolved fluorescence detection utilising two photon excitation in microfluidic separation systems

该项目的重点是开发一种新的方法，用于在基于芯片的分离系统中对微量样品进行无标记检测。这将通过与发射光的时间分辨荧光检测相结合的双光子荧光激发来实现。与深UV光谱区域中的常见单光子激发相比，这应该能够在低于300 nm不透明的经济芯片材料（例如硼硅酸盐玻璃或塑料）中进行无标记检测。 荧光寿命的动态测定将用于芯片电泳中的可靠峰分配。本文将系统地研究微流控系统中双光子激发的潜力，并设计一个高性能的荧光激发和检测系统。最后，开发的系统和方法将在几个应用领域进行评估，如生物分析，药物分析和集成有机合成。

This project focuses on the development of a novel approach for label-free detection of tiny sample amounts in chip-based separation systems. This will be achieved by two photon fluorescence excitation combined with time resolved fluorescence detection of the emission light. In comparison to common single photon excitation in the deep UV-spectral region, this should enable label free detection in economic chip materials which are not transparent below 300nm, such as borosilicate glass or plastics. On the fly determination of fluorescence life times will be utilized for reliable peak assignment in chip electrophoresis. The potential of two photon excitation in microfluidic systems will be evaluated systematically with of a high performance system for fluorescence excitation and detection. Finally the developed systems and methods will be evaluated in several application areas, such as Bionanalytics, drug analysis and integrated organic synthesis.