Investigation of Daphnia as a genetic model organism

这种无处不在的淡水微型甲壳动物被分析为监测生态系统完整性的模式动物。水蚤既可以克隆繁殖，也可以进行有性繁殖，这使它们非常适合进行遗传学研究，但胚胎的体外发育和基因修饰技术的建立尚未建立。我们调查了一种自然雄性繁殖的条件，发现了水蚤品系，其性别完全由日长决定。此外，我们还建立了显微注射和后续胚胎体外发育的最佳条件。目前的研究是初步的，但我们的发现为进一步的研究提供了有用的知识，为进一步的遗传研究提供了依据。

The ubiquitous, freshwater microcrustacean Daphnia has been analyzed as a model animal for monitoring ecosystem integrity. Daphnia are capable of either clonal or sexual reproduction, making them ideally suited for genetic research, but the establishment of in vitro development of the embryos and gene modification techniques has not been established. We investigate a condition for natural male production and found the Daphnia strain whose sex is solely regulated by day length. In addition, we established an optimal condition for microinjections and the following in vitro development of the embryos. The current research is preliminary but our findings provide useful knowledge for further study for genetic study for Daphnids.