Reverse vaccinology for cell-mediated immunity

本研究旨在寻找影响利什曼病疫苗抗原有效性的参数。统计分析显示氨基酸组成、表达水平和外泌体的定位是影响参数。基于这三个参数的公式从整个蛋白质组中区分出先前表征的疫苗抗原。此外，使用该公式鉴定的新抗原与已知抗原具有相同的抗原性。这些结果表明，所开发的计算机方法可用于鉴定利什曼病候选疫苗抗原。

Identification of parameters influencing the effectiveness of vaccine antigens for leishmaniasis was searched in this study. Statistical analyses revealed amino acid composition, expression level, and localization to exosome were influential parameters. A formula based on the three parameters discriminated the previously characterized vaccine antigens from the whole proteome. Furthermore, novel antigens identified using the formula were as antigenic as the known antigens. These results suggest the in silico approach developed is useful to identify candidate vaccine antigens for leishmaniasis.