Development of imaging changes in microenvironment with in the sentinel lymph node combined with micrometastasis of carcinoma cells

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Metastasis of carcinoma cells mainly occurs through the lymphatic system, and the extent of metastasis in lymph nodes is clinically used as a useful prognostic indication. The clinical importance of SLN has been proven in many breast cancer patients. However, the biological and histological properties of lymphatic endothelial cells (LEC) in the SLN that interact with micrometastatic carcinoma cells remain unclear. Therefore, using the human breast carcinoma cell line MDA-MB-231, we have attempted to examine the effects of supernatants cultured with the cell line on the expression of adhesion molecules on human LEC and then to investigate whether the expressed adhesion molecules accelerate the attachment of carcinoma cells to the LEC. The experimental findings suggest that MDA-MB-231 may release or leak ATP, which produces the overexpression of ICAM-1 on human LEC through activation of purinergic receptors and then facilities ICAM-1-mediated attachment of carcinoma cells to the LEC.