Development of angiogenic therapy with human Muse cells

从培养的人脂肪组织源性干细胞/基质细胞（hASCs）中分离出SSEA-3+多能间充质细胞（Muse细胞）并对其进行表征，并评估其治疗糖尿病性皮肤溃疡的潜力。与缺乏muse的ASCs相比，富含muse的ASCs分别表现出多能性和细胞增殖基因的上调和下调。1型糖尿病SCID小鼠产生皮肤溃疡，与非糖尿病SCID小鼠相比，伤口愈合延迟。与使用缺乏muse的细胞相比，使用富含muse的细胞治疗可显著加速伤口愈合。移植细胞分别以表皮角质形成细胞和血管内皮细胞的形式融入再生表皮和真皮。然而，在周围的完整区域没有检测到它们。因此，选择的ASCs群体在加速与1型糖尿病相关的受损伤口愈合方面具有更大的治疗效果。

SSEA-3+ multipotent mesenchymal cells (Muse cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Muse-rich ASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared to Muse-poor counterpart. Skin ulcers were generated in SCID mice with type 1 diabetes, which showed delayed wound healing compared to non-diabetic SCID mice. Treatment with Muse-rich cells significantly accelerated wound healing compared to treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated epidermis and dermis as epidermal keratinocytes and vascular endothelial cells, respectively. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound healing associated with type 1 diabetes.