Small regulatory RNAs from the halophilic archaeon Haloferax volcanii

虽然细菌和真核生物中的小调控RNA的功能和机制已经被详细分析，但关于调控RNA在生命的第三个领域--古生代中的出现和功能知之甚少。在上一次资助期间，我们用三种不同的实验方法和两种生物信息学方法鉴定了嗜盐古细菌Haloferax Volcanii的小RNA(SRNA)种群。总共确定了200多个潜在的调控RNA。此外，我们可以证明，删除sRNA基因是可行的，而且在大多数情况下不是致命的。在下一个资助期，我们的主要目标是确定已确定的潜在调控sRNA的目标。这将使我们能够详细地描述sRNA-靶相互作用的特征，并确定古代sRNA调控的机制。

While the function and mechanism of small regulatory RNAs in Bacteria and Eukarya have been analysed in detail, not much is known about the occurence and function of regulatory RNAs in the third domain of life, the Archaea. In the last funding period, we identified the small RNA (sRNA) population of the halophilic archaeon Haloferax volcanii with three different experimental and two bioinformatic methods. Taken together more than 200 potential regulatory RNAs were identified. In addition, we could show that deletion of sRNA genes is feasible and in most cases not lethal. In the next funding period, our main goal is to identify targets of the identified potential regulatory sRNAs. This will allow the characterisation of the sRNA-target interaction in detail and to identify the mechanism of archaeal sRNA regulation.