Screening of food factors that target the quality control system of mitochondria

我们筛选了促进自噬降解的食物因素，从而导致线粒体质量控制。在HeLa细胞中，木犀草素及其衍生物显著促进自噬适配蛋白p62的自噬降解。相反，在J774.1巨噬细胞中，经黄酮醇如槲皮素处理后，p62的mRNA表达显著增强，而木犀草素衍生物处理后未见明显影响。这些结果强烈表明，多酚类物质诱导自噬的活性与细胞类型有关。转录因子Nrf2的激活增强了p62mRNA的表达。此外，用黄酮醇处理J774.1细胞后，细胞内和细胞外的自噬底物IL-1β水平均下降。这些结果表明，多酚诱导自噬可能是细胞内蛋白质和细胞器质量控制的一种很好的策略。

We screened the food factors that enhance the autophagic degradation leading to mitochondrial quality control. In HeLa cells, luteolin and the derivatives significantly enhanced the autophagic degradation of p62, an adaptor protein of autophagy. In contrast, in J774.1 macrophages, the mRNA expression of p62 was significantly enhanced upon treatment with flavonols such as quercetin, whereas no effects were observed upon treatment with luteolin derivatives. These results strongly suggest that the activities of polyphenols for autophagy induction are dependent on cell types. It was also found that p62 mRNA expression was enhanced through the activation of Nrf2, a transcription factor. Furthermore, the pretreatment of J774.1 with flavonols resulted in the decrease in both intracellular and extracellular IL-1beta, an autophagy substrate. These results suggest that autophagy induction by polyphenols might be good strategy for the quality control of intracellular proteins and organelles.